Abstract
The aim of this study was isolation of the inclusion body hepatitis virus (IBH) from infected commercial layer hens in Ninevah governorate and identification the pathological effects of this virus. The natural infection of IBH revealed pathognomic basophilic and eosinophilic intranuclear inclusion bodies in the nuclei of many hepatocytes of infected chickens. Haemagglutination test of the allantoic fluid show titer 3Log2 of the isolated virus after one passage in the chiken embryos with 1% rats washed RBCs. The experimental infection is done by injection of layers and breeders at 20 weeks age by liver homogenate from infected chickens and revealed the same pathognomic gross and histopathological lesions.
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