This study aimed to investigate the effect of chronic use for 2 month with omega-3 on the level of neurobehavioral and motor activity in the open field. The study showed an effect for different doses of omega-3 on the nervous system and behavior when drainage drug by mouth, that are easily hand to deal with the rats dosage with 10, 50, 250, 500 mgkg of body weight. Rats in doses 10, 50, 250, 500, 1000 mg/kg recorded a significant decrease in number of crossed squares and the number of rearing comparison with the control group. Pocking test recorded significant increase in the number of times introduction of head in the holes compared to the control group in doses of 50, 250, 500 mgkg of body weight, a dose of 1000 mgkg showed a prolongation in the time required to avoid animal high edge, with a lower score swimming and stretching in a period of rotation in the negative geotaxis test compared with the control group, while the rest of the doses did not show any significant difference compared with control. In test of tonic immobility response all the doses recorded a significant decrease in the stillness and freeze for rats movement, compared with control group. We concluded that omega-3 has beneficial effect on the level of neurobehavioral and motor activity in the open field activity in addition to development cognitive behavior of animals, except dose 1000 mgkg Shaw some behavioral difference compare with control group.