Abstract

The present study was designed to investigate the effect of Vitamin E 600 mg/kg diet, Vitamin C 450 mg/kg diet and sodium selenite 0.5 mg/kg diet in adult white Leghorn male chickens (30 weeks), which were concomitantly exposed to oxidative stress induced by hydrogen peroxide (0.5%) supplemented with drinking water for 6 weeks on blood picture. Blood samples were collected at (0, 3, 6) weeks of treatment. Hydrogen peroxide caused a significant increase in the total leukocyte count, heterophils percentage and heterophils /lymphocytes ratio (stress index) accompanied with a significant increase in liver malondialdehyde level associated with significant decrease in hemoglobin concentration, packed cell volume, lymphocyte and esoinophil percentage. It also caused a significant decrease in liver glutathione on the 6th week of the treatment compared with control group. The antioxidants with hydrogen peroxide caused a significant decrease in the total leukocyte count, heterophils percentage and stress index accompanied with a significant decrease in malondialdehyde level in liver tissue compared with hydrogen peroxide alone and control group, beside that a significant increase in hemoglobin concentration, packed cell volume, lymphocyte, esoinophil percentage and liver glutathione level compared with hydrogen peroxide alone which returned to control group values. In general, treatments with Vitamin E, C and Sodium selenite reversed the adverse effects produced by hydrogen peroxide on certain physiological parameters in adult male chickens.