• Register
  • Login
  • العربیة

Iraqi Journal of Veterinary Sciences

  1. Home
  2. Identification of beef using restriction fragment length polymorphism–polymerase chain reaction

Current Issue

By Issue

By Subject

Keyword Index

Author Index

About Journal

FAQ

Peer Review Process

News

Aims and Scope

Editorial Board

Editorial Staff

Publication Ethics

Indexing and Abstracting

Related Links

Identification of beef using restriction fragment length polymorphism–polymerase chain reaction

    Author

    • R. A. Al-Sanjary
,
10.33899/ijvs.2009.5687
  • Article Information
  • Download
  • Export Citation
  • Statistics
  • Share

Abstract

To differentiate the beef from other types of meat consumed by human, DNA markers based on polymerase chain reaction restriction fragment length polymorphism technique is performed by using universal primers designed on mitochondrial cytochrome b gene to obtain amplified band 359 bp, then digested with some of restriction enzymes like Tru91, RsaI, Hinf I, Hae III, Alu I, Taq I, Mob I. The result revealed that, the Hinf I enzyme produce three bands 198, 117, 44 bp and the Hae III enzyme revealed two band 285, 74 bp, the Alu I enzyme also produced two band but the molecular weight are 190, 169 bp. The other enzymes did not reveal any digestion of the amplified bands and this result is a characteristic unique to beef compared with other types of meat when using same enzymes.

  • XML
  • PDF 0 K
  • RIS
  • EndNote
  • Mendeley
  • BibTeX
  • APA
  • MLA
  • HARVARD
  • VANCOUVER
    • Article View: 521
    • PDF Download: 796
Iraqi Journal of Veterinary Sciences
Volume 23, Issue 1
June 2009
Page 43-46
Files
  • XML
  • PDF 0 K
History
  • Received: 01 June 2009
  • Revised: 20 June 2009
  • Accepted: 25 June 2009
  • Published: 28 June 2009
Share
Export Citation
  • RIS
  • EndNote
  • Mendeley
  • BibTeX
  • APA
  • MLA
  • HARVARD
  • VANCOUVER
Statistics
  • Article View: 521
  • PDF Download: 796

APA

A. Al-Sanjary, R. (2009). Identification of beef using restriction fragment length polymorphism–polymerase chain reaction. Iraqi Journal of Veterinary Sciences, 23(1), 43-46. doi: 10.33899/ijvs.2009.5687

MLA

R. A. Al-Sanjary. "Identification of beef using restriction fragment length polymorphism–polymerase chain reaction". Iraqi Journal of Veterinary Sciences, 23, 1, 2009, 43-46. doi: 10.33899/ijvs.2009.5687

HARVARD

A. Al-Sanjary, R. (2009). 'Identification of beef using restriction fragment length polymorphism–polymerase chain reaction', Iraqi Journal of Veterinary Sciences, 23(1), pp. 43-46. doi: 10.33899/ijvs.2009.5687

VANCOUVER

A. Al-Sanjary, R. Identification of beef using restriction fragment length polymorphism–polymerase chain reaction. Iraqi Journal of Veterinary Sciences, 2009; 23(1): 43-46. doi: 10.33899/ijvs.2009.5687

  • Home
  • About Journal
  • Editorial Board
  • Submit Manuscript
  • Contact Us
  • Glossary
  • Sitemap

News

  • IJVS in the first rank during Iraqi Science day 2023-01-30

 

© 2023, College of Veterinary Medicine, University of Mosul

Newsletter Subscription

Subscribe to the journal newsletter and receive the latest news and updates

© Journal Management System. Powered by ejournalplus