This study was conducted to evaluate the application of the massage method of the uterus and ovaries by rectal palpation to treat true anoestrus in local Iraqi buffaloes. The study was conducted during the period from September 2007 to May 2008, on 236 buffalo cows including 113 multiparous and 123 heifers. Multiparous buffalo cows were divided into treated group (n=63) and control group (n=50), heifers were divided in to treated group (n=73) and control group (n=50). Results of the present study indicated a significant effect of the massage method for the uterus and ovaries in heifers by producing oestrus (80.82%), as well as a significant effect of treatment on multiparous buffalo cows was observed (58.73%). The response of heifers to treatment with massage method was significantly higher than multiparous buffalo cows. It could be concluded that using manual massage for uterus and ovaries by rectal palpation is a successful method for treating true anestrous in buffaloes.