The aim of the study was to determine the prevalence of Toxoplasma gondii in native donkeys in Mousl, Iraq. Fifty two sera (9 males and 43 females) were examined by Latex agglutination test, Modified latex agglutination test with 2- mercaptoethanol test and Indirect ELISA test (Indirect IgG ELISA). The prevalence of Toxoplasma gondii in native donkeys was 46.15 %. Acute cases 8.33% and chronic cases 91.67 % when differentiated by Modified latex agglutination test with 2- mercaptoethanol test. The percentages of female and male infections were 51.16% (22/43) and 22.22% (2/9), respectively by using latex agglutination test, and the titeration of antibodies ranged between 1:20 - 1:1280 and for Indirect IgG ELISA it was 22.72% positive cases.