This study was designed to investigate the effect of laser treatment on thyroid functions and hormones in female rabbits. A total of 18 female rabbits were used in this investigation. Six rabbits were used for examining the thyroid gland location and dissected to be sure that laser therapy will be directed to this gland. Twelve female rabbits were used in this experiment to study the physiological effect of laser therapy (4J/cm2) on normal thyroid function they were divided into two equal groups, six used as control and the others were exposed to laser therapy at the thyroid gland region for three successive days for 5 minutes on each side. Then blood was collected for hormonal assay from ear vein at 1, 6, 12, 18, 24 and 30 days after the last dose of laser treatment. The results revealed a gradual decrease in serum T3 and T4 levels with a concomitant increase in TSH level at the first eighteen days of laser treatment, then normalization of hormones level were take place to be normal after thirty days. More work is needed on this subject to examine the specific role of laser on glands.