This research was conducted to explore the effect of rosella (Hibiscus sabdariffa) on female rats on oxidative stress which induced by 0.5% H2O2. Oxidative stress has been investigated via tissue (aorta and heart) malonadyaldehyde (MDA) as indirect lipid peroxidation index. For atherosclerotic lesions follow up light microscopical technique has been applied. The result elucidate significant reduction in lipid profit parameters namely: low density lipoprotein (LDL-c), triglycerides, very low density lipoprotein (vLDL-c), atherogenic index and significant elevation in high density lipoprotein (HDL-c) in few animals treated with H2O2 and rosella extract, parallely, this research illustrate reduction in aorta and heart MDA concentration, concomitant with significant rising in glutathione (GSH) level. Histopathologically, this study revealed fatty streaks associated with infiltration of mononuclear inflammatory cells have been detected after 60 days, in animal treated with rosella revealed reduction in lipid vacuoles and proliferation in vascular smooth muscle cells (VSMcs) in media toward intimal layers after 40 days from treatment.