The aim of the present study was to examine the acute and sub acute toxicity of ciprofloxacin on lipids metabolism of chicks which included determination of cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, and albumin levels in serum of chicks. The biochemical changes induced by giving ciprofloxacin as a single dose (200 and 400 mg/kg. body weight intraperitoneally) included significant increases of cholesterol, triglyceride and low density lipoprotein levels in serum, whereas albumin level significantly decreased, and there was no significant changes in high density lipoprotein levels as compared with control group. Repeated treatment with ciprofloxacin (100 mg/kg. body weight intra peritoneal) for 14 days caused significant increase in cholesterol level, albumin level significantly decreased as compared with control group, whereas it did not change significantly high density lipoprotein and triglyceride levels, repeated treatment of ciprofloxacin also showed significant decrease of the body weights of the chicks as compared with control group. The results suggest that there are toxic effects of ciprofloxacin on lipids metabolism as seen through changes in cholesterol, triglyceride, albumin and low density lipoprotein level.