This study was applied on six donkeys and two horses. The castration was performed by making longitudinal skin incision in area above to the level of the head of the epididymis at the site of the panpniform plexus and by blunt dissection the spermatic cord was isolated from other surrounding tissues without opening of tunica vaginalis. At the same time moderate pressure was applied on the scrotum by the other hand to expel the testis from the incision. The spermatic cord crushed and ligated at the site of crushing, and the testis was removed by cutting the spermatic cord below the ligation to ensure that there were no bleeding from the stump of spermatic cord. The site of operation was closed by three rows. The first row included the area of passage of the spermatic cord and the cavity of scrotum, while the second included subcutaneous tissues with simple continuous suture by using catgut materials for both rows. The skin closed with simple interrupted suture by using silk. The results revealed presence of slight swelling at the site of operation, the animals returned to work early and quickly. Healing occurred with minimal complications.