Abstract
One day old broiler chicks from four groups of broiler breeder chickens were immunized by different routes of vaccination. (1: orally), (2: intraocular), (3: a single injection of oil emulsion vaccine), (4: unvaccinated). The first two groups were vaccinated with live NDV vaccine. Serological antibody titers were determined to study the correlation between the different groups using ELISA test and leukocyte migration inhibition test to study the cell-mediated immune response in different groups. By results of this study vaccination of one day old chicks is very important to enhance the maternal derived antibody response also the cell-mediated immunity play an important role in increase the level of immunity beside the humeral one.