Abstract

The aim of this study was to know the effect of microwave, infrared and ozone effect on the pathogenicity and pathogensis of Cryptosporidium sp. in fish. 50 common carp (Cyprinus carpio) have been used which is randomly divided into five group 10 fish at each group. The first group was negative control the second one positive control inoculated with the LD₅₀ of the parasite oocyct, the third one inoculated with treated oocycte with microwave of 50 wave length for 15 minute the forth group are inoculated with oocyct treated with infra red wave at 700-750 anglistome wave length and 20 watt/m3 absorbion, the fifth group inoculate with oocycts treated with Ozone at concentration of 20 PPM. The result showed no clinical signs and pathological lesions have been seen in all group expect the control which show dullness and no external stimulate response
from the forth day of infection. Examination of fecal samples showed the control one which inoculated with LD₅₀ of oocycts which showed the presence of the oocycts in their feces and increase the shedding of the oocystsin feces with progression of the experiment time until to the fifteen day. Histopathological examination showed no lesions in the fish intestine in all groups except the control group which inoculated with the LD₅₀ of oocycte showed different stage of parasite replication at the intestinal villi with sloughing and necrosis of some of these villi with mucinus degeneration and adhesion of other villi. In conclusion the treatment of Cryptosporidium sp. oocysts with microwave and Infra red and Ozone with criteria mention above lead to distruction the infectious ability and pathogencity in tissues for this parasite in common carp through prevention of any clinical signs and pathological lesion (Gross and Microscopy) at the section taken from the intestine of these fish