Abstract

The study was conducted for evaluation of two routes of administration of Br. melitensis strain Rev.1 vaccine include subcutaneous injection and conjunctival dropping with two doses standard dose (1-2×10⁹ colony forming units CFU) and reduced dose (1×10⁷ CFU), in the production of antibodies titers and its interference with serological tests. The study was included (20) of native ewes with Ages between 1.5-4 years, divided into four equal groups blood samples was collected prevaccination to ensure from Brucella antibodies free. Then vaccine administered and blood samples were collected during 1^st, 2^nd, 3^rd, 4^th, 5^th and 6^th months postvaccination. The results revealed that injection of vaccine via s/c route with 1-2×10⁹CFU dose causing interference with serological tests used (Rose Bengal, indirect ELISA and competitive ELISA tests) they showed positive results of all samples during the period of study whereas the interference with results of serological tests reduced when the vaccine administered in reduced dose, the results of vaccine administration via conjunctival route showed reduction in interference with results of used serological tests especially when administered in reduced dose in comparison with administered via s/c route the results showed that specificity of c-ELISA test was 11,11% and 66,66% when vaccine administered via s/c route with two doses standard and reduced respectively, while the specificity of i-ELISA was zero, whereas the administration of vaccine via conjunctival route with standard and reduced doses the specificity of c-ELISA and i-ELISA was 87.5% and 93.33%, zero, and 60% respectively. The results also showed that vaccine administered via s/c route with standard dose was the better in which the resulting antibody titers and its duration in comparison with administration via conjunctival route with same dose.