Abstract
Tow hundred milk samples were collected from healthy appearance ewes and ninety five samples from clinical infected ewes with mastitis from deferent flocks distributed in the middle region of Syria from January to June 2008, isolation and biochemical identification testes of Mycoplasma were applied to all samples. Results showed that fourteen samples were positive (12.63%) for clinical infected ewes and 1% for healthy appearance ewes. Biochemical tests revealed that three isolates positive to Mycoplasma capricolum and eleven isolates to Mycoplasma agalactiae.