Abstract

This study was included 18 of native lactating ewes divided into three equal groups which was vaccinated with Rev.1 vaccine. The sensitivity of Rose Bengal test (RB) was 50% ,50% and 0% in the 4^th day postvaccination in animals of 1^st group which vaccinated with dose 1×10⁹ colony forming units(CFU)/ml via subcutaneous route, the 2^nd group vaccinated with dose 1×10⁹ CFU/ml via conjunctival route the 3^rd group vaccinated with dose 1×10⁷ CFU/ml via subcutaneous route respectively, then increased to reach 100% in days 7, 30 and 15 in vaccinated animal groups 1^st, 2^ndand 3^rd respectively and continue till the final of experiment, the sensitivity of serum agglutination test (SAT) reached 100% in 7^th and 15^th day in 1^st and 3^rd groups respectively, while, it reached the highest value 80% in 30^th day in 2^nd group. The milk ELISA showed sensitivity reached 100% from the starting of experiment till the final in the 60 day postvaccination in all vaccinated groups, the results of SAT revealed the highest antibody titers was in the 1^st and 3^rd then 2^nd groups. also showed significant correlation between optical density (O.D.) values of milk ELISA and the results of SAT while the correlation coefficient value was r= 0.80 at the level P<0.05. The results of bacterial culture was negative for milk and vaginal swabs of ewes in all vaccinated groups.