Abstract

This study were designed to differentiate various domestic animals raw milk (Buffalo, Cow, Sheep, Goat) by using Species Specific Polymerase Chain Reaction technique. The technique was applied in this study for 1^st time in our country in milk hygiene field in an attempt to protect the health of Iraqi consumers and their economics. The average quality of genomic milk's DNA extract from (10) animals of each Species were (180-240) microgram / 10 ml while purity was about (1.75-1.88) of all extracted DNA. The study shows that there was a different amplified bands between different studied animal species and weighted (520) bp for Buffalo; (365) bp for Cow; (275) bp for sheep and (400) bp for goat compared with standard bands of the DNA Ladder on (1.2%) of agarose gel by using UV Transilluminator apparatus. The results of this study indicated that there is a possibility of using polymerase chain reaction, species specific PCR markers to differentiate different types of raw milk awing to different animals, due to the accuracy and high sensitivity of this technique. The technique asses in preventing adulteration of milk and milk product and also to protect consumers for health and economic reason.