Abstract

Sheep pox virus was isolated from seven various regions in Nineveh governorate from cases infected with sheep pox virus with different ages and both sex. These isolates were Cultivated on Chorioallantoic membrane of embrynated chicken eggs, one of these isolate showed typical lesions (pock lesion), death of embryo, while the other isolates did not showed any lesions in embryonated chicken eggs. The diagnosis of the isolate were done by neutralization of the isolate with hyper immune serum of sheep pox virus and convalsant serum in the embryonated chicken eggs the result show decrease the titer when neutralized with hyper immune serum of sheep pox virus and higher decrease in the titer of isolate when neutralized with the convalsant serum. To known the capability of the growing isolate for induce pathogenecity, the isolate was injected intradermally in lambs, the isolate showed cutaneous lesions in different regions in the body with increasing of body temperature and death. The isolate gave high titer of antibody in the experimental infection by using serum neutralization test.