College of Veterinary Medicine / University of Mosul
  • Register
  • Login
  • العربیة

Iraqi Journal of Veterinary Sciences

Notice

As part of Open Journals’ initiatives, we create website for scholarly open access journals. If you are responsible for this journal and would like to know more about how to use the editorial system, please visit our website at https://ejournalplus.com or
send us an email to info@ejournalplus.com

We will contact you soon

  1. Home
  2. Volume 36, Issue 3
  3. Author

Current Issue

By Issue

By Subject

Keyword Index

Author Index

Indexing Databases XML

About Journal

Aims and Scope

Editorial Board

Editorial Staff

Facts and Figures

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Rapid diagnosis of leishmania spp. in blood samples using gold nanoparticles.

    Musafer H. Al-Ardi

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 3, Pages 587-590
10.33899/ijvs.2021.130985.1906

  • Show Article
  • Download
  • Cite
  • Statistics
  • Share

Abstract

There are many methods to diagnose Leishmania spp. a microscopic, serological or molecular methods. However, finding a fast and accurate diagnostic assay is a necessary and urgent requirement nowadays. here, we improved nano detection method to diagnose Leishmania spp. In blood samples. In this study, we used a probe of oligonucleotide sequences associated with AuNPs (20 nm) to diagnose different Leishmania spp. in blood samples of dogs. For this purpose, three series replicates of 10 μl of 23 ng / μl of Leishmania spp. DNA are used. A change in the solution colour to red is an indicator of a positive result, while the change to purple is a negative result. Then we compared these results with the molecular assay of (IST1) gene. The overall relative sensitivity of the result is (90%). The result shows that 10 out of 10 samples of visceral Leishmania are positive with relative sensitivity and specificity (100%). Eight out of 10 samples of cutaneous leishmania are positive with relative sensitivity and specificity (80%). The present method is a fast and accurate method for detecting small amounts of DNA, and it is easy to distinguish by visual inspection.
Keywords:
    DNA,, ,،,؛Nanotechnology,, ,،,؛AUNPs,, ,،,؛Leishmania spp.,, ,،,؛rapid diagnosis
Main Subjects:
  • Veterinary Protozoa
  • PDF (453 K)
  • XML
(2022). Rapid diagnosis of leishmania spp. in blood samples using gold nanoparticles.. Iraqi Journal of Veterinary Sciences, 36(3), 587-590. doi: 10.33899/ijvs.2021.130985.1906
Musafer H. Al-Ardi. "Rapid diagnosis of leishmania spp. in blood samples using gold nanoparticles.". Iraqi Journal of Veterinary Sciences, 36, 3, 2022, 587-590. doi: 10.33899/ijvs.2021.130985.1906
(2022). 'Rapid diagnosis of leishmania spp. in blood samples using gold nanoparticles.', Iraqi Journal of Veterinary Sciences, 36(3), pp. 587-590. doi: 10.33899/ijvs.2021.130985.1906
Rapid diagnosis of leishmania spp. in blood samples using gold nanoparticles.. Iraqi Journal of Veterinary Sciences, 2022; 36(3): 587-590. doi: 10.33899/ijvs.2021.130985.1906
  • RIS
  • EndNote
  • BibTeX
  • APA
  • MLA
  • Harvard
  • Vancouver
  • Article View: 462
  • PDF Download: 218
  • LinkedIn
  • Twitter
  • Facebook
  • Google
  • Telegram
  • Home
  • Glossary
  • News
  • Aims and Scope
  • Privacy Policy
  • Sitemap

 

© 2023, College of Veterinary Medicine, University of Mosul

 
This journal is licensed under a Creative Commons Attribution 4.0 International (CC-BY 4.0)

Powered by eJournalPlus