This study for the evaluation safety of uses propolis at (1%, 2.5%, 5%, 10%) concentrations (alone and synergistically with antibiotic) inside the intramammary the lactation period of the healthy ewes, compared with treated and control groups. The result of safety test indicated that the uses of propolis as intramammary infusion inside the teat cause some effects that disappeared later, like elevation of SCC in milk, decrease in milk yield, prolong residue time for propolis, in addition to the hardness in the consistency of the udder and enlargement the supramammay lymph node when compared with treated and control groups. Also there is significant difference between groups in milk composition. The second trial was to evaluate the Pharmacological activity of propolis preparation and synergism preparation and compared it with antibiotic and control groups in treatment of experimentally induced Staphylococcus aureus Mastitis in the udder of ewes, after confirmed the diagnosis of Staphylococcus aureus strains by Api 20 Staph, one isolate of Staphylococcus aureus was selected based on (its resistance to 10 of 12 antibiotic tested). The infected dose 5 cfu/ml of bacterial suspension was selected to experimentally induced Staphylococcus aureus mastitis in 25 ewes, After the appearance of infection 18 -72 hours post inoculation of bacterial suspension of Staphylococcus aureus in the udder by intramammary route in ewes, the ewes treated paraenterally by antibiotic, antiinflammatory and antipyretic drug and locally in four groups (propolis 1% and 10%) (8 ewes) which appeared very mild side effects, synergism between propolis and antibiotic (4 ewes), antibiotic (12 ewes) and (1 ewe) as control group. The results showed systemic cure in all ewes but with differences in the local clinical cure between groups according to the type of antibiotic and its company production without bacterial cure in all groups , so that make the ewes under the risk of reinfection under any stress condition.