Abstract

Isolation and identification of the species related to actinomycetes which have a role in Hypersensitvity Pneumonitis (HP). A total of (241) samples were collected from different sources including 83 sputum samples, 83 blood samples from the workers in cows, sheep poultry farms, poultry slaughter house, textuary, flour, tannery factories, sawmills, and hospitalized patient as well as 25 samples from the worker environment (soil, air, water), through August 2008 to February 2009. The isolates were identified to species level depending on morphological, biochemical and physiological tests, including the species Nocardia spp, Rhodococcus equi, Saccharomonospora viridis, Nocardia nova, Coryneformbacteria were isolated in high percentage from individual with normal level of IgE 100 IU\ml with and without clinical symptoms, and with less frequency the species Nocardia spp, Citrococcus spp, Coryneformbacteria in individual with high level of IgE > 100 IU \ ml. The species Saccharomonospora viridis, Nocardia nova, Nocardia spp. also were isolated from individual with high level of IgG.> 25 IU \ ml compared with whom have normal level of IgG 25 IU \ ml. were the species Saccharomonospora viridis, Coryneformbacteria, Nocardia spp, Citrococcus spp, Rhodococcus equi, isolated. But not isolated from the control.