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Thin layer chromatographic investigation of some veterinary drugs

    Authors

    • Zena S. Hamed 1
    • Basil M. Yahya 2

    1 Department of Pharmacology and Toxicology, College of Pharmacy. University of Mosul, Mosul, Iraq

    2 Department of Pharmacology, College of Pharmacy, University of Mosul, Mosul, Iraq

,

Document Type : Research Paper

10.33899/ijvs.2012.168712
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Abstract

A simple technique, using solvent extraction and thin layer chromatography for the detection and separation of a number of commonly prescribed veterinary drugs was studied. Blood and urine have been considered as possible biological samples taken from the animals. The extraction of each sample was done according to the type of the drug as it is acidic or basic, then the extract was spotted on TLC plate by capillary tube after lining the starting line first. Separations of the components of the samples were developed after running the plate in a tight lid tank containing solvent (mobile phase). The identification of separated spots were carried out after lining the end line for the solvent front then by spraying the plate with mercurous nitrate reagent for acidic drugs and acidic iodoplatinate or potassium permanganate reagent for basic drugs. The rate of flow value (Rf) for each compound was measured and the color of spots were determined. This work provides a simple and rapid qualitative technique for the detection of veterinary drugs used at therapeutic doses and in overdosage.

Keywords

  • Thin layer chromatography
  • Veterinary drugs
  • Drug analysis

Main Subjects

  • Veterinary Pharmacology and Toxicology
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Iraqi Journal of Veterinary Sciences
Volume 26, Suppl. II - Issue Serial Number 3
September 2012
Page 53-55
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  • PDF 177.58 K
History
  • Received: 01 June 2012
  • Revised: 01 July 2012
  • Accepted: 01 August 2012
  • Published: 01 September 2012
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APA

Hamed, Z. S., & Yahya, B. M. (2012). Thin layer chromatographic investigation of some veterinary drugs. Iraqi Journal of Veterinary Sciences, 26(Suppl. II), 53-55. doi: 10.33899/ijvs.2012.168712

MLA

Zena S. Hamed; Basil M. Yahya. "Thin layer chromatographic investigation of some veterinary drugs". Iraqi Journal of Veterinary Sciences, 26, Suppl. II, 2012, 53-55. doi: 10.33899/ijvs.2012.168712

HARVARD

Hamed, Z. S., Yahya, B. M. (2012). 'Thin layer chromatographic investigation of some veterinary drugs', Iraqi Journal of Veterinary Sciences, 26(Suppl. II), pp. 53-55. doi: 10.33899/ijvs.2012.168712

VANCOUVER

Hamed, Z. S., Yahya, B. M. Thin layer chromatographic investigation of some veterinary drugs. Iraqi Journal of Veterinary Sciences, 2012; 26(Suppl. II): 53-55. doi: 10.33899/ijvs.2012.168712

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