This study included measuring of immune response to oily inactivated Avian Influenza vaccine(AIV) serotype H9N2 injected alone in broiler at 1 day old (group1), also the measuring of Newcastle disease vaccine (NDV) Avenue VG/GA strain when given orally at 7and21 days old (gp2), then study the immune response interaction between these vaccines at same time (gp3), as well as the measuring of maternal immunity of both AIV and NDV vaccines (gp4), for this study 160 broiler chicks were used and ELISA test was used for detecting the antibodies in the serum of all groups. Results exhibited immune response to AIV (gp1) at 14, 21, 28, 35, 42 days post vaccination at the titer of 27.3, 28.2, 29.2, 46.2, 47.3, ELISA unit (EU), respectively. (gp2) show the formation of ND antibodies at 14 days post vaccination with titer of 31.3 EU which decline to 27.3 EU at 21 days, then elevated to 40.5, 44.1 EU at 28, 35 days respectively, then decline to 36.3 EU at 42 days post vaccination. The results of (gp3) show decline and irregular antibodies titers for both AIV and NDV in comparison with (-ve) control group. The results of (gp4) showed intermediate level of maternal antibodies against NDV but not detected against AIV. In conclusion, there was negatively immune response interaction between AIV and NDV when vaccinated in same broiler flock.