This study involved a disclosure of Aflatoxin M1 in 50 samples of thick cream (Gaymer), 30 were manufactured from raw buffalo milk and 20 were collected from local markets of different regions in Mosul city, by using Enzyme Linked Immunosorbent Assay (ELISA) method. The results revealed that 90% of the manufactured thick cream were positive to residual AFM1, with a mean value of 16.164 ng/Kg. Only 3.3% of the manufactured thick cream samples had a concentration values above the permissible limits. Within the same subject, local thick cream samples showed a higher AFM1 level than the manufactured samples with a mean of 29.158 ng/Kg and 15% of local thick cream samples had higher AFM1 than the permissible limits according to European Commission. (i.e 50 ng /Kg).