The present study aimed to investigate the histological changes of Ganoderma lucidum (Red Reishi mushroom) on female and male reproductive system by using light microscope. Fifteen white rat weighing 200-250g was used in the experiments. The animals was divided mainly into three groups and these groups were subdivided into 3 groups for female, and 3 groups for male, that’s group represented as control group without treatment (G1). The 2nd received only 0.03 gm and vitamin C diluted with D.W. and the 3rd group received 0.03 gm of Ganoderma lucidum . The results of the present study showed that there are no side efects of Ganoderma lucidum on female and male genital system of rats (positive effects).