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First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province/ Iraq

    Salah Mahdi Karim Khalefa Ali Mansour Ali Hassan Janabi Nawras K. M. Al-Nakeeb

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 123-126
10.33899/ijvs.2019.125525.1047

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Abstract

This study was initiated for the first time for identification, using sequencing and phylogenetic analyses, of pseudocowpox PCPV that inhabit dairy cows in Al-Qadisiyah province, Iraq. Scab sampling was performed to obtain specimens from udder and teats of 18 affected cows. Initially, a polymerase chain reaction (PCR) method was followed to target a 408-bp piece of the GM_CSF/IL-2 inhibition factor gene (GIF) that belongs to PCPV. Then, the PCR products were sent out to partial sequencing of the GIF gene. The results of the PCR have indicated the presence of the virus in only 3 out of 18 samples. When the sequences were studied using phylogeny, the results have revealed that one of our PCPV strains has a close matching with some of the world strains such as from New Zealand. While two of the current study strains have clustered together with a strain from Finland. The results of our study confirm the presence of the PCPV in dairy cows that induces milker’s nodules.
Keywords:
    Dairy cows <i>GIF</i> gene PCR Pseudocowpox virus Sequencing
Main Subjects:
  • Veterinary Virology
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(2019). First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province/ Iraq. Iraqi Journal of Veterinary Sciences, 33(1), 123-126. doi: 10.33899/ijvs.2019.125525.1047
Salah Mahdi Karim; Khalefa Ali Mansour; Ali Hassan Janabi; Nawras K. M. Al-Nakeeb. "First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province/ Iraq". Iraqi Journal of Veterinary Sciences, 33, 1, 2019, 123-126. doi: 10.33899/ijvs.2019.125525.1047
(2019). 'First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province/ Iraq', Iraqi Journal of Veterinary Sciences, 33(1), pp. 123-126. doi: 10.33899/ijvs.2019.125525.1047
First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province/ Iraq. Iraqi Journal of Veterinary Sciences, 2019; 33(1): 123-126. doi: 10.33899/ijvs.2019.125525.1047
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