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The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef

    Authors

    • Saba Falah Klaif 1
    • Hassan Naser 2
    • Jenan N. Sadeq 2

    1 Unit of Zoonotic Disease Research, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq

    2 College of Veterinary Medicine, University of Al-Qadissyiah University, Al-Qadissyiah - Iraq.

,

Document Type : Research Paper

10.33899/ijvs.2019.125531.1053
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Abstract

The present study aimed to describe the genetic relationships of zoonotic characterization of Klebsiella pneumoniae isolated from Human urinary tract and beef. The study includes (50) urine samples from human and (50) beef samples. The isolation and identification of Klebsiella pneumonia were done by using enrichment culture method and Vitek 2, then confirmed by PCR technique based on 16S ribosomal RNA gene which designed in this study using NCBI-GenBank (LT599801.1) and DNA sequencing was done on some positive isolates. The results show that Klebsiella pneumoniae was isolated from Beef at 38(76%) And from human at 32(64%) by vitek2. The PCR technique was show highly sensitive and specific confirmative detection of Klebsiella Pneumonia isolates at Clarify DNA sequencing of a partial sequence of 16S ribosomal RNA gene was shown homology sequence identity highly with NCBI-Blast Klebsiella pneumoniae isolates. The phylogenetic analysis was show clear genetic similarity at (0.5 genetic change) between human and beef in Klebsiella pneumoniae isolates. The gene sequence deposited into NCBI-GenBank accession numbers (MF314450.1, MF314451.1, MF314452.1, MF314453.1). In conclusion, the study presents the first report in Iraq of genetic relationship among K. pneumoniae isolates from beef and humans. Therefore, it is essential to define the role of animals as an important source for the distribution of pathogen related to public health.

Keywords

  • <i>Klebsiella pneumonia</i>
  • 16s rRNA
  • Polymerase change reaction
  • Phylogenetic tree

Main Subjects

  • Veterinary Microbiology
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Iraqi Journal of Veterinary Sciences
Volume 33, Issue 1
April 2019
Page 75-80
Files
  • XML
  • PDF 486.63 K
History
  • Received: 18 May 2018
  • Revised: 20 June 2018
  • Accept: 22 July 2018
  • Publish: 31 March 2019
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  • Article View: 961
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APA

Klaif, S. F., Naser, H., & Sadeq, J. (2019). The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef. Iraqi Journal of Veterinary Sciences, 33(1), 75-80. doi: 10.33899/ijvs.2019.125531.1053

MLA

Saba Falah Klaif; Hassan Naser; Jenan N. Sadeq. "The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef". Iraqi Journal of Veterinary Sciences, 33, 1, 2019, 75-80. doi: 10.33899/ijvs.2019.125531.1053

HARVARD

Klaif, S. F., Naser, H., Sadeq, J. (2019). 'The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef', Iraqi Journal of Veterinary Sciences, 33(1), pp. 75-80. doi: 10.33899/ijvs.2019.125531.1053

VANCOUVER

Klaif, S. F., Naser, H., Sadeq, J. The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef. Iraqi Journal of Veterinary Sciences, 2019; 33(1): 75-80. doi: 10.33899/ijvs.2019.125531.1053

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