The present research was designed to study the effect zinc sulphate (15 mg/kg/orally) and cadmium chloride (25 mg/L) with drinking water on the level of Heat shock protein 70 , testosterone and luteinizing hormone in adult male rabbits for six weeks. Twenty adult male rabbits randomly divided to four groups of five males per group: control group, the cadmium chloride group, the zinc sulphate with cadmium chloride together group and zinc sulphate group alone. Blood samples were withdrawn every two weeks of experiment. To investigate Heat shock protein 70, testosterone and luteinizing hormone by using ELISA technique. The result show that treatment of male rabbits with cadmium chloride resulted in a significant increase in HSP70 in 6th week, accompanied by a significant decrease in the level of testosterone and luteinizing hormone 2nd, 3rd, 6th week compared to control group, supplement with zinc sulphate and cadmium chloride together resulted in a significant decrease in HSP70 in 6th week with a significant increase in testosterone and luteinizing hormone in 2nd, 4th and 6th, week compared to the cadmium chloride group. The treatment with zinc sulphate alone caused a significant decrease in HSP70 in 6th week and significant increase in the level of testosterone in 2nd, 4th, and 6th week and luteinizing hormone in 6th week compared with cadmium chloride group. We concluded that has an effect on the level of Heat shock protein and male sex hormones and that giving zinc with cadmium together reduced the effects of cadmium as well as zinc supplementation improve the negative effect on the level of sex hormone in adult male rabbits.