Newcastle disease is a highly contagious disease of domestic and wild birds result in huge economic losses due to extreme morbidity and mortality. The aim of this paper is to explore the possibility of the harmful impact of Newcastle disease vaccine on certain biochemical profiles regarded to chicken liver. This study conducted during January-April 2014, one day old - 53 chicks were divided into two groups; vaccinated (with Newcastle disease vaccine) group at the age of 1, 3, and 7 days and unvaccinated group. Results show damaging effects of vaccination on the liver glycogen and malondialdehyde concentrations, serum superoxide dismutase, catalase, glutathione peroxidase, gamma-glutamyl transferase, alanine transaminase, and aspartate transaminase activities. In contrast, studied parameters look to return to their usual range at the age of 30 days. From the convincing outcome of our investigation, it can be concluded that Newcastle disease vaccination has a detrimental influence on the liver of chickens, nonetheless this effect can be overwhelmed by the time.