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Iraqi Journal of Veterinary Sciences

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Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue

    M. Hasoon

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 2, Pages 119-125
10.33899/ijvs.2019.153837

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Abstract

In this study, pieces of skin from common carp (Cyprinus carpio L.) fish were collected freshly from juveniles from a privet fish farm in Duhok city, Kurdistan-Iraq. Those pieces of skin were cultured In Vitro by using two methods of explanting; direct explants and enzymatic pre-digested explants of tissue fragments. The results showed a highest cell yield (5×103 cell/ml) on passage 2 of enzymatic pre-digested tissues and higher cell growth rate in compare with cell yield (38×102 cell/ml) gained from direct explant tissues. Both procedures showed forming of clumped cells colonies in initial passages (P1 and P2), and the morphological changes were developed in both cells to show a fibroblast like appearance thereafter. Such findings are important and advantageous to suggest a faster and more efficient protocol for tissue culture cells isolation with lower cost and better isolation success as well.
Keywords:
    Tissue culture Isolation Method Fish Skin, Enzymatic Explants
Main Subjects:
  • Animal Physiology
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(2019). Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue. Iraqi Journal of Veterinary Sciences, 32(2), 119-125. doi: 10.33899/ijvs.2019.153837
M. Hasoon. "Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue". Iraqi Journal of Veterinary Sciences, 32, 2, 2019, 119-125. doi: 10.33899/ijvs.2019.153837
(2019). 'Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue', Iraqi Journal of Veterinary Sciences, 32(2), pp. 119-125. doi: 10.33899/ijvs.2019.153837
Evaluation of the isolation protocols of primary cells from common carp (Cyprinus carpio L.) skin tissue. Iraqi Journal of Veterinary Sciences, 2019; 32(2): 119-125. doi: 10.33899/ijvs.2019.153837
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