Genotyping of cystic echinococcosis isolates from clinical samples of human and domestic animals
Iraqi Journal of Veterinary Sciences,
2016, Volume 30, Issue 2, Pages 33-39
AbstractCystic hydatid disease is a cosmopolitan important disease in both human and animals. Many strains were investigated in this parasite. The aim of study was to characterize genotype variations of Echinococcus granulosus isolates collected from human and domestic animals in Al-Qadisiyah province/ Iraq based on sequencing of nad1 mitochondrial gene. Eighty hydatid cysts of human (12), sheep (15), cattle (36), and camels (17) were collected from hospital and slaughter house of the province, during October 2014 to June 2015; microscopic examination was made for cysts fluid to determine the fertility. DNAs extraction was done for each sample in addition to purify and concentrate of extracted DNA samples was performed to determine nad1 (400bp) gene used conventional PCR method. Phylogenetic analysis was performed using NCBI-Blast Alignment identification and Unweighted Pair Group Method with Arithmetic Mean. Twenty five (10 from human and 5 from each studied animals) samples were chosen due to their fertility and high DNA purity, in which three strains (genotypes) were investigated including sheep strain (G1) 40%, buffalo strain (G3) 48% and camel strain (G6) 12%, where human samples related to G1(20%) and G3(80%); sheep samples related to G1(80%) and G3(20%); cattle samples related to G1(60%), G3 (20%) and G6 (20%); camels samples related to G1(20%), G3(40%) and G6(40%). The dominant strain is a buffalo strain (G3); both of buffalo strain (G3) and sheep strain (G1) represented the actual source of human infection. There is no host specificity of detected genotypes.
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