This study was carried to isolate and identificate of Enterococcus. faecalis from small intestine and cecum of quails by culturing on differential and selective media. The concentration of E. faecalis suspension was fixed for experimental infection. Quails divided randomly into four groups, the first group considered as control group, the other groups injected with 0.5 ml of bacterial suspension as following: second group 1X108 CFU, the third group injected with 1X109 CFU, and the forth group injected with 1X1010 CFU. The clinical signs and pathological changes of heart, liver and kidney were observed at 3, 7, 14 and 21 days after infection. The results showed identification of E. faecalis after culturing and isolation of it. The gross lesions represented by opacity of pericardium, heart hypertrophy and liver infarction, histopathological lesions include beginning of endocarditis, severe fatty changes with localized recent thrombus and severe necrosis in liver, and cell swelling of epithelium lining renal tubules and apoptosis in kidney. The histopathological changes were more severe at 3 and 7 days post infection. This study concludes that quails have a strong defense and immune mechanism despite the appearance of pathological changes with high concentrations of bacterial suspension which cause death in other animals such as mice and rats, also E.faecalis possesses the ability to induce apoptosis.