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Using of competitive ELISA in detection of brucella antibodies in cattle sera

    Authors

    • O. Kh. Al-Hankawe
    • O. M. Al-Iraqi
    • M. I. Al-Farwachi
    • M. O. Abdul-Majeed
,
10.33899/ijvs.2009.5739
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Abstract

Competitive ELISA test was used in this study to identify brucella antibodies in cattle sera in Mosul, and assessing the results that are given by Rose-Bengal test. Results of testing 126 cattle sera revealed that the seroprevalence was 16.7% using C-ELISA and 18.3% using RBT. There were differences in seroprevalence according to sex of animals, females showed higher seroprevalence than males, it was 19.1% and 20.2% in females and 9.4% and 12.5% in males by C-ELISA and RBT respectively. Also there were differences in seroprevalence according to age of animals and pregnancy status of females. Kappa value revealed that there was little agreement between C-ELISA and RBT, (Kappa value was 0.229). RBT was not accurate in giving real seroprevalence, since there were 13 false-negative samples from 21 samples positive with C-ELISA, and there were 15 false-positive samples from 105 samples negative with C-ELISA. Results of tube agglutination test and 2-Mercaptoethanol test indicated a decline of titers from those of previous studies, lower titer of TAT was 1/10 (19.05%) and most of titers were lower than 1/40 (76.2%) in 2-Me test. This study concluded that the brucella antibodies in cattle sera in Mosul was increased from previous years and most of cases were acute.

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Iraqi Journal of Veterinary Sciences
Volume 23, Issue 2
December 2009
Page 97-103
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History
  • Received: 01 December 2009
  • Revised: 20 December 2009
  • Accepted: 25 December 2009
  • Published: 28 December 2009
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APA

Kh. Al-Hankawe, O., M. Al-Iraqi, O., I. Al-Farwachi, M., & O. Abdul-Majeed, M. (2009). Using of competitive ELISA in detection of brucella antibodies in cattle sera. Iraqi Journal of Veterinary Sciences, 23(2), 97-103. doi: 10.33899/ijvs.2009.5739

MLA

O. Kh. Al-Hankawe; O. M. Al-Iraqi; M. I. Al-Farwachi; M. O. Abdul-Majeed. "Using of competitive ELISA in detection of brucella antibodies in cattle sera". Iraqi Journal of Veterinary Sciences, 23, 2, 2009, 97-103. doi: 10.33899/ijvs.2009.5739

HARVARD

Kh. Al-Hankawe, O., M. Al-Iraqi, O., I. Al-Farwachi, M., O. Abdul-Majeed, M. (2009). 'Using of competitive ELISA in detection of brucella antibodies in cattle sera', Iraqi Journal of Veterinary Sciences, 23(2), pp. 97-103. doi: 10.33899/ijvs.2009.5739

VANCOUVER

Kh. Al-Hankawe, O., M. Al-Iraqi, O., I. Al-Farwachi, M., O. Abdul-Majeed, M. Using of competitive ELISA in detection of brucella antibodies in cattle sera. Iraqi Journal of Veterinary Sciences, 2009; 23(2): 97-103. doi: 10.33899/ijvs.2009.5739

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