Iraqi Journal of Veterinary Sciences

Abstract

In order to isolate Bovine Herpes virus type -1- (BHV-1) from cattle using cell culture (Bovine fetal kidney cell) and diagnosis by neutralization test,  592 swabs (230 Nasal swabs,  102 Vaginal swabs, 230 Ocular swabs, 12 swabs from Balanus and penises, 8 swabs from skin lesion in udder and 10 Anal swabs) were collected from animals suffering from respiratory signs, ocular discharge with congestion in conjugtiva and opacity in the eye, abortion in different stages of pregnancy with vaginal discharge,  inflammation in balanus and penis with discharge in ox older than 2 years,  some cattle suffering from mastitis with chronic skin lesions in udder and teat,  some young calves suffer from bloody diarrhea, and healthy cattle. The result of isolation and diagnosis of virus showed (30.57%) total percentage of infection using cell culture and (21.62%) using neutralization test. The highest percentage of infection was in Jub ramla farm, and the lowest percentage infection was in Dura farm using both methods. The study also showed the high percentage in vaginal swabs (49.01%) in cell culture, and in neutralization test (34.31%), whereas the lowest percentage of infection appeared in swabs from balanus and penises and swabs from skin lesion in udder (25%) in cell culture, while the lowest percentage of infection (25%) appeared in ocular swabs using neutralization test. The study detected the high percentage of infection in young calves (less than 6 months) of both sex by using both methods. The results of propagation of samples in cell culture appear one blind passage except anal swabs (two blind passage), the cytopathic effect (CPE) differ in appearance as the time decrease with progress passage. The CPE manifested by cell swelling shrink and rounded of cells. Cluster appearance and Vacuoles. The CPE variad in passage depending on type of sample, the highest potency was in balanus and penises swabs when compared with other samples.