Comparative study on the effect of cadmium and hydrocortisone treatment on the brain of rats experimentally infected with Toxoplasma gondii

Karam Hashim Al-Mallah¹, Sura Salim Aghwan² and Qaes Talb Al-Obaidi³

1Department of Pathology and Poultry Diseases, 2Department of Microbiology, 3Department of Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, Iraq

karamyahya74@uomosul.edu.iq, 0000-0002-8191-100, (corresponding author)

sura.aghwan@uomosul.edu.iq, 0000-0002-9907-8742  

qaestalb1976@uomosul.edu.iq, 0000-0002-1334-3745

2020-09-22

2021-01-23

Abstract

This study was dedicated to detecting the effects of cadmium chloride and of cortisone on experimental infection with Toxoplasma gondii in rats through studying brain tissue. Twenty-four adult albino male rats were used, divided into four groups, comprising: untreated control as group 1, group 2: infected with intraperitoneal injection of Toxoplasma gondii 80 tissue cysts per animal, group 3: infected with Toxoplasma gondii and treated with intraperitoneal injection of cadmium chloride 1.5mg/kg once a week, group 4: infected with Toxoplasma gondii and treated with intramuscular injection of hydrocortisone in a dose 0.5 mg/kg once a week. After 30 days of treatment for all groups, the animals were sacrificed, and the gross and histopathological examinations were performed on the brains of the rats. The results revealed the presence of changes in the infected groups including appearance of toxoplasma tissue cysts in the grey matter, with mild to moderate perineuronal and periaxonal edema. There were further changes observed in infected groups which treated with cadmium chloride including perineuronal satelletosis, perineuronal edema and demyelination in neuronal axons, otherwise the infected groups treated with cortisone did not show any noticeable difference from the group of infection only. It is concluded that cadmium increase the intensity of occurrence of lesions induced by Toxoplasma gondii in the brain tissue of the rats, considering that all noticeable lesions ranged from mild to moderate in severity, and the differences between groups may be related to the nature of the pathogen used.

Keywords: Toxoplasma gondii, Cadmium chloride, Hydrocortisone, rats

دراسة مقارنة لتأثیر المعاملة بکل من الکادمیوم والهایدروکورتیزون فی أدمغة الجرذان المخمجة تجریبیاً بالمقوسات الکوندیة

کرم هاشم الملاح¹، سرى سالم أغوان² و قیس طالب العبیدی³

¹فرع الأمراض وأمراض الدواجن، ²فرع الأحیاء المجهریة، ³فرع الطب الباطنی والوقائی، کلیة الطب البیطری، جامعة الموصل، الموصل، العراق

الخلاصة

لمعرفة تأثیر کل من کلورید الکادمیوم والکورتیزون على الخمج التجریبی بطفیلی المقوسات الکوندیة من خلال دراسة نسیج الدماغ فی الجرذان تم تصمیم الدراسة الحالیة. استخدم فیها 24 من ذکور الجرذان البیضاء البالغة، قسمت الى 4 مجامیع، مجموعة سیطرة غیر معاملة، مجموعة الخمج بطفیلی المقوسات الکوندیة اعطیت الخمج بحقن 80 کیس نسیجی فی الخلب لکل حیوان، ومجموعة مخمجة بطفیلی المقوسات ومعاملة بکلورید الکادمیوم بحقنه فی الخلب بجرعة 1.5 ملغم/کغم مرة واحدة فی الأسبوع، ومجموعة مخمجه بطفیلی المقوسات ومعاملة بعقار الهایدروکورتیزون بحقنه فی العضل بجرعة 0.5 ملغم/کغم مرة واحدة فی الأسبوع. بعد 30 یوما من المعاملة لکل مجامیع التجربة تمت التضحیة بالحیوانات واجراء الفحص المرضی العیانی والنسجی على ادمغة الجرذان . اظهرت النتائج وجود تغیرات فی نسیج الدماغ للمجوعة المحقونة بطفیلی المقوسات الکوندیة حیث لوحظت الاکیاس النسیجیة للطفیلی فی اللب الدماغی مع وجود وذمة طفیفة حول المحاور العصبیة، بینما کانت هناک تغیرات إضافیة للمجموعة المعاملة بکلورید الکادمیوم تمثلت بتجمع الخلایا النجمیة حول الخلایا العصبیة مع وجود الوذمة حول الخلایا العصبیة وازالة النخاعین فی المحاور العصبیة، بینما لم تظهر المجموعة المخمجة والمعاملة بالکورتیزون اختلافا عن المجموعة المخمجة بالطفیلی فقط. لقد تم استنتاج أن اضافة الکادمیوم یزید من شدة التغیرات المرضیة التی یحدثها طفیلی المقوسة الکوندیة فی حین لا یؤثر اعطاء عقار الکورتیزون على شدة الإصابة للفترة المستخدمة للتجربة. وان التغیرات المرضیة الملاحظة کانت من طفیفة الى متوسطة الشدة. وان اختلاف التغیر فی الصورة النسجیة قد یعود للخصوصیة الامراضیة لکل من العاملین.

Introduction

Toxoplasmosis is zoonotic parasitic disease (1,2) can be considered as one of the most universally distributed infectious diseases (3). The infection is caused by Toxoplasma gondii, which is a protozoal parasite from the order: Sporozoasida, family: Sarcocystiidae, genus: Toxoplasma (4). This parasite can invade several mammalian tissues (5,6), as well as avian tissues like in local poultry industry (7) Toxoplasma tissue cysts can develop in lungs, liver and kidneys but is most commonly noticed in nervous and muscular tissues, including brain, eyes, skeletal muscles, and heart (8,9). The lesions induced by Toxoplasma infection are typically accompanied by inflammatory response, especially the necrosis in the infected tissues generating clinical signs such as anemia, headache, fever, and muscle pain as well as long-term complications resulting from specific cells destruction in brain, eyes, lungs and heart (10). The cadmium is one of the heavy metals of industrial importance, but it is also a dangerous pollutant in environment which the exposure to it leading to certain toxic effects to many tissues, especially the brain (11,12). The serious toxic effect of cadmium on plants and animals is caused by its ability to inactivate the intramembranous ionic exchange, thus affecting their permeability (13), and as a result cadmium has a direct harmful effect on the liver and kidneys and may result in acute renal failure (14), It is also reported that cadmium chloride consumption affects the endocrine functions with adverse impact on the histological view of each thyroid and adrenal glands of rats (15). The cortisone drugs are glucocorticoids, which are steroidal compounds with a hormonal effect used clinically to suppress inflammations and exaggerated immune responses by stopping the release of inflammatory mediators (16). The goal of the study is to compare the pattern of Toxoplasma infection in the brain of rats, alone, and in combination with cadmium chloride exposure and under effect of cortisone drug to monitor the effects through histopathological point of view.

Materials and methods

Animals and housing

Twenty-four adult albino male rats aged from 2.5 to 3 months and weighing between 200 - 250 gm, were used in this experiment. They were obtained from the animal house of the college of Veterinary Medicine after receiving the approval of the Ethical Committee for the experiment. The animals were kept in standard plastic cages of 12*25*10 cm, with steel buckle cover, and were freely supplied with food and water and housed within 22±25ºC and 12-12 light-dark cycle. Those animals were randomly distributed between four groups with six animals for each group.

The isolation of Toxoplasma gondii

Toxoplasma gondii was diagnosed by examining the impression smears made from placentas of the aborted ewes (17). The positive samples for toxoplasmosis were digested by pepsin as described by (18). the fluid extracted from infected placenta was inoculated in albino mice intraperitoneally, typical tissue cysts were found in the brain of the inoculated mice who sacrificed and the suspension was made from infected brains by homogenisation and washing with phosphate buffered saline three times, this suspension containing the brain tissue cysts was inoculated in the peritoneal cavity of the rats of experiment.

The experiment and manipulation

The first group was the control group, which was not subjected to any treatment for 30 days; the second group was infected with 80 Toxoplasma gondii tissue cyst for animal as an infective dose by intraperitoneal injection once, on day one of the experiment. The third group was infected and treated with cadmium chloride 1.5 mg /kg by intraperitoneal injection once a week. The fourth group was also infected and treated with hydrocortisone 0.5 mg/kg once a week for the period of the experiment.

Gross and histopathological examination

The animals were sacrificed, dissected and the gross pathological changes were recorded; then samples were taken from the brain and directly preserved in 10% neutral buffered formalin. Trimming was performed followed by dehydration using gradual concentrations of ethyl alcohol, cleared with xylol, embedded with paraffin wax at 57ºC, set in paraffin blocks and sectioned to 4-6 micrometer sections and stained with hematoxylin and eosin (19-21), and examined under light microscope Kruss, Germany and photographed using digital camera, Sony, Japan.

Results

Histopathological examination revealed the existence of pathological changes in brain tissue at the three treated groups compared with control group. The toxoplasma infected group showed toxoplasma tissue cyst in brain tissue, mostly in the cerebral medulla surrounded by mild microgliosis, perineuronal and periaxonal edema (Figure 1). In the infected and cadmium chloride-treated group, the same lesions mentioned above were observed with perineuronal satelletosis, Hyperaemic arterioles and capillaries, perineuronal edema and mild microglial cells proliferation with increase of degenerative and necrotic neurons surrounded by microglial cells (Figure 2). The infected group with cortisone treatment showed the same lesions as the infected group with noticeable toxoplama tissue cyst and cerebral cortex and medulla with microgliosis, perineuronal and periaxonal edema (Figure 3).

Figure 1: A: Microscopic image of tissue cysts in impression smears of the brain of rat (Black arrows). Giemsa stain, magnification 1000 X. B: Section of the brain medulla of the rat from the group infected with Toxoplasma gondii showing Toxoplasma tissue cyst (Black arrow) with mild periaxonal edema (Red arrows). H&E stain. Magnification 780 X.

Figure 2: A: Section of brain cortex of rat infected with Toxoplasma gondii and treated with cadmium chloride showing congestion of capillaries (Black arrow) with mild peri axonal edema (Red arrow) Magnification 780 X. B: Section of brain Medulla of rat infected with Toxoplasma gondii and treated with cadmium showing neuronal demyelination (Black arrow) Perineuronal microgliosis (Red arrow). 730 X. C: Section of brain medulla of rat infected with Toxoplasma gondii and treated with cadmium sowing capillary congestion (Black arrow), Perineuronal edema (Red arrow). Magnification 115X. D: Section of brain medulla of rat infected with Toxoplasma gondii and treated with cadmium sowing demyelination and vacuolation in myelin sheaths (Black arrow), microgliosis (Red arrow). Magnification 145 X. Staining H&E for all images.

Figure 3: A: Section in brain cortex section of rat infected with Toxoplasma gondii and treated with cortisone showing toxoplasma tissue cyst (Black arrow) with mild peri axonal edema (Red arrow). Magnification 780 X. B: Section of brain medulla of rat infected with Toxoplasma gondii and treated with cortisone showing vasogenic edema (Black arrow) with mild demyelination in myelin sheathes (Red arrow). 730 X. Staining H&E for all images.

Discussion

The Toxoplasma gondi infected group revealed histopathological changes in brain tissue represented by the presence of tissue cyst at cortex and medulla of cerebrum and medulla oblongata. Similar results were obtained by Al-Khalidy et al (22) in Al-Diwaniyah province/ Iraq, who noticed the calcified Toxoplasma tissue cysts in brain of affected cats. This study also demonstrated pathological changes as mild focal microgliosis, perineuronal and periaxonal edema, agreeing with Al-Saidya and Al-Kennany (23), who recorded these lesions after experimental infection with local isolates of Toxoplasma gondii in local cats and they also reported encephalitis in more advanced stages. Our results also were supported by the findings of Al-Khanaq and Jasim (24), who stated that intraperitoneal inoculation of Toxoplasma gondii in albino male rats resulted in the development of perineuronal edema, Demyelination, Microgliosis. Besides, pathological changes were noticed in rats’ brains of cadmium-controlled group compared with control, including perineuronal microgliosis and astrocytosis, hyperemic capillaries, and mild to moderate perineuronal and periaxonal edema. There was also increase in number of apoptotic neurons surrounded by microglial cells, while similar results were reported by Wang and Du (25), who showed that prolonged exposure to cadmium generated accumulative toxic effects in liver, kidneys, central and peripheral nervous systems resulting from precipitation of cadmium and increased permeability of blood brain barriers. It was stated by Manton and Cook (26) that the mechanism of neurotoxicity with heavy metals is not completely understood but the anatomical and physiological nature of choroid plexus makes it a target for xenobiotics like cadmium and lead that accumulates more intensively than in cerebrospinal fluid or cerebral cortex. Observations by Kaoud and Mekawy (27) supported the behavioral effects of cadmium reflecting nervous effects as dose-dependence increases in non-social activity and decreases the social activity in rats, including nose to genital, nose to nose contacts and frequency of fighting. Disabled cerebral functions by neurotoxicity were confirmed by histopathological and neurochemical analysis.Yang and Fan (11) stated that severity of histological changes in brain in cadmium toxicity depends on the dose by noticing more advanced changes at medium and high doses than noticed in low dose of cadmium. The sever changes were separation of gray matter in cerebral cortex, severe hyperemia, infiltration of eosinophils, vacuolar demyelination and increased number of apoptotic neurons. The same researchers described ultrastructural changes including polar aggregation of heterogenous chromatin, incomplete or fused nuclear membrane, Perinuclear areas expansion, abnormal folding in mitochondrial membranes and decreased synaptic cleft areas, so they concluded that cadmium causes microscopical and ultrastructural changes in infant mice cerebral cortex. Similar results were obtained by Afifi and Embaby (28) through giving rats 5 mg /Kg B.W of cadmium. The cerebral cortex revealed severe degenerative changes in pyramidal and granular cells and ultrastructural changes including rough endoplasmic reticulum and Golgi apparatus expansion, mitochondrial swelling and Polar orientation of nuclear chromatin besides demyelination in neuronal axons and vacuolation of neuropil, and they noticed less severe changes when animals were treated with ascorbic acid, giving indication of the protective rule of the antioxidants against cadmium neurotoxicity. It was stated by Borges et al (29) that cadmium increases lipid peroxidation and levels of reactive oxygen species in neural plates. This was supported by Yuan et al (30) who explained that incidence of neuronal apoptosis was connected to ROS production because of disturbances in calcium metabolism and disruption of mitochondrial membranes activity, resulting in activation of caspas-3, caspas-9, and PARP concluding that apoptosis being activated through mitochondrial -caspase signaling pathway and this may explain the histopathological changes in our study.

Conclusion

Cadmium intense the occurrence of lesions induced by Toxoplasma gondii in the brain tissue of the rats, considering that all noticeable lesions ranged from mild to moderate in severity, and the variance between groups may be related to the nature of the pathogens.

Acknowledgements

We would like to convey our thanks to the College of Veterinary Medicine, University of Mosul, and to the workers in the Animal House for their support.

Conflicts of interest

The authors declare no conflict of interests with regard to the manuscript.