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Molecular differentiation of Thysaniezia (Helictometra) giardi and Moniezia species based on 18s rRNA gene in small ruminants

    Authors

    1 Department of Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq

    2 Department of internal and preventive veterinary medicine, College of Veterinary Medicine, University of Al Qadisiyah, Iraq

    3 Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq

    4 Department of microbiology and parasitology, college of veterinary medicine, Al-Qadisiyah university, Iraq

    5 Dep. of pathology and poultry diseases , college of veterinary medicine, University of Al-Qadisiyah

,

Document Type : Research Paper

Abstract

This study was conducted to investigate Anoplocephalidia Cestoda in sheep and goat and evaluate the 18s rRNA to genetically differentiate the genera of this family. Sixty sample tapeworms were collected from small intestines of 30 sheep and 30 goats from different slaughterhouses in Al-Najaf and Al-Qadisiyah provinces, during September, 2016 to February, 2017. Based on polymerase chain reaction (PCR) and 18s rRNA gene partial sequencing (18sGPS) methods used, tapeworm infection of sheep and goat’s intestines was 32.9% and 31.4%, respectively. The partial gene sequencing of the 18S rRNA gene showed two closely related isolates of M. benedeni which are aligned distinctly to an NCBI isolate of the same species from China. For T. giardia, the outcomes of the phylogenetic analysis unveiled three distinct local isolates which were similar to an NCBI database isolate from China. The current data ensure the importance of the molecular techniques in differentiating between Thysaniezia (Helictometra) giardi and Moniezia species that were identified for their presence in the small intestines of sheep and goats.

Keywords

Main Subjects

Highlights

1- There are a closely related isolates of M. benedeni which are aligned distinctly to an NCBI isolate of the same species from China.

2- For T. giardia, the outcomes of the phylogenetic analysis unveiled three distinct local isolates which were similar to an NCBI database isolate from China.

3- The current data ensure the importance of the molecular techniques in differentiating between Thysaniezia (Helictometra) giardi and Moniezia species that were identified for their presence in the small intestines of sheep and goats.

References
 
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Iraqi Journal of Veterinary Sciences
Volume 35, Issue 1
January 2021
Page 105-108
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History
  • Received: 15 December 2019
  • Revised: 13 January 2020
  • Accept: 20 January 2020
  • Publish: 01 January 2021
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