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Genetic detection to Aeromonas hydrophila proteolytic activity in milk samples (cows, buffaloes and goats) in Basra governorate

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 253-258
10.33899/ijvs.2019.125888.1174

Abstract

This study aim is to determine the incidence and the virulence of Aeromonas hydrophila in raw milk, randomly collected from Basra governorate by using of polymerase chain reaction (PCR) technique. In this study, the total number of raw milk samples collected from cows, buffaloes and goats that kept from different the regions of Basra governorate were 90 samples. The PCR technique is modern method which regarded as a reliable tool to detect virulent gene of the A. hydrophila isolates. The PCR assays using the primers sets SerAh-F and SerAh-R resulted in the amplification of 650-bp bands from the targeted proteases gene of the A. hydrophil. The result of the present study showed that the results of PCR concerning the proteolytic activity of A. hydrophila in the tested raw milk samples according to animals' source. The higher percentage of the proteolytic activity was found in the cow's raw milk samples 40% and in the buffalo's milk samples was 26.7% while, the proteolytic activity did not find in the goat's milk samples. The association between the source of the milk sample and proteolytic A. hydrophila positive results was considered to be statistically highly significant. The higher percentage of the A. hydrophila isolates found in the raw cow milk was 40%, and the A. hydrophila isolates found in the raw buffalo milk was 26.7%, while, the A. hydrophila isolates did not find in the goat milk.
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Main Subjects:
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  1. Fernández-BravoA,JoséFiguerasM.AnUpdateontheGenus Aeromonas: Taxonomy,Epidemiology,andPathogenicity.microorganismsJ.2020;8(129):2-39.https://doi:10.3390 microorganisms8010129.  
  2. AsmaaT, Heba A,Rasha MM, Attia A, Ibrahim E, Abeer EA. Molecular characterisation, genotyping and survival of Aeromonas hydrophila isolated from milk, dairy products and humans in Egypt. Inter Dairy J.2016;63:52-58.https://doi: 10.1016/j.idairyj.2016.07.011
  3. Subashkumar R, Thayumanavan T, Lakshmanaperumalsamy P.RAPD and ERIC-PCR typing of virulent Aeromonas hydrophila isolated from children with acute diarrhoea. J Cell MolBio. 2014;1(1):12. http://jcmb.halic.edu.tr
  4. Liang X,Zhaoxu M,Lanwei Z,Yanhua C, Xue H,Huaxi Y.The diversity and proteolytic properties of psychrotrophic bacteria in raw cows’ milk from north China. Int Dairy J. 2016;66:34-41. http://dx.doi.org/10.1016/j.idairyj.2016.10.014
  5. Alperi A, Figueras MJ. Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli. Clin Microbiol Infect. 2010;16(10):1563-7.https:// doi: 10.1111/j.1469-0691.2010.03203.x
  6.  Zhang S, Li H,UlukoH, LiuL,PangX,LvJ. Investigation of protease production by Pseudomonas fluorescens BJ-10 and degradation on milk proteins. J Food Proc Pres. 2015;39:2466-2472. https://doi.org/10.1111/jfpp.12496 
  7.  Alberto C,Javier Y, Alejandro T,Maria SA,Carmen H, Jose A,German N.A Major secreted elastase is essential for pathogenicity ofAeromonas hydrophila.Infect Immuol.2000;68(6):3233-3241. https:// doi: 10.1128/IAI.68.6.3233-3241.2000
  8. FreitasAC, NunesMP, MilhomenAM,RicciardiID. Occurrence and characterization of Aeromonas spp. in pasteurized milk and white cheese in Riode Janeiro Brazil. J Food Pro.1993;56(1):62-65.https:// doi/pdf/10.4315/0362-028X-56.1.62
  9. NagwaA, Salah A, Madeha A, Magdy S. Enumeration and characterization of Aeromonas spp isolated from milk and some dairy products in Sharkia governorate Egypt.Alexandria J Vet Sci.2014;40:52-64. https://doi.org/10.5455/ajvs.49073
  10. Bramley A,Mckinnon C. The microbiology of raw milk. New York: Elsevier; 1990. 163-208 p. https://doi.org/10.1002/0471723959.ch2
  11. Sorhaug T, Psychrotrophic V. Total bacterial count in bulk milk samples. Vet Med Czech.1992; 47(8): 227-233 . https:// doi.org/ 10. 17221 /5829-vetmed
  12. Sørhaug T, Stepaniak L. Psychrotrophs and their enzymes in milk and dairy products: Quality aspects Trends in Food Science & Technology J. 1997;8(2):35-41. https://doi.org/10.1016/S0924-2244(97)01006-6
  13. Machado SG,Bazzolli DMS, Vanetti MCD. Development of a PCR method for detecting proteolytic psychrotrophic bacteria in raw milk. Int Dairy J.2013;29:8-14. https://doi.org/10.1016/j.idairyj.2012.09.007
  14. Taylor R,Weinstein M, BrynerH. Campylobacter fetus infection in human subjects association with raw milk. Am J Med.1979;66:779-783. https://doi.org/10.1016/0002-9343(79)91116-1
  15. ArcuriF, SilvaL, Brito P, BritoF, LangeC, MagalhaesA. Counting, isolation and characterization of psychrotrophic bacteria from refrigerated raw milk. Ciencia Rural.2008;38:2250-2255. https://doi.org/10.1590/s0103-84782008000800025
  16. Delgado S, RachidC, FernandezE, RychlikT, AlegriaA, PeixotoS. Diversity of thermophilic bacteria in raw, pasteurized and selectively-cultured milk, as assessed by culturing, PCRDGGE and pyrosequencing. Food Microbiol.2013; 36:103 -111. https://doi.org/ 10.1016/j.fm.2013.04.015
  17. RasolofoA, LaPointeG, Roy D. Assessment of the bacterial diversity of treated and untreated milk during cold storage by T-RFLP and PCRDGGE methods. Dairy Sci Technol.2011;91:573-597.https:// doi: 10.1007/s13594-011-0027-4
  18. Ling L, John J, Ling F, Qingkun Z, Yan T, Li Huang, Daxi R, Pan Y.Characterization of the indigenous microflora in raw and pasteurizedbuffalo milk during storage at refrigeration temperature by high-throughput sequencing.JDairy Sci.2016; 99(9):7016-7024. https://doi.org/10.3168/jds.2016-11041
  19. Ahmed M,Hoda Z,Nibal H, Nihad M.Phenotyping, virulence characteristics ofAeromonas species and the effect ofessential plant oils as antimicrobial agents againstpathogeneticsisolate from different source.Am J Infect Dis.2014;10 (1):21-35.https:// doi:10.3844/ajidsp.2014.21.35
  20. Sechi A, Deriu A, Falchi P, Fadda G, Zanetti S. Distribution of virulence genes in Aeromonas spp. isolated from Sardinian waters and from patients with diarrhoea. J Appl Microbiol.2002;92:221-227. https://doi.org/10.1046/j.1365-2672.2002.01522.x
  21. BedeltavanaA, HaghkhahM,NazerA. Phenotypic characterization and PCR-ribotyping of Pseudomonas fluorescens isolates, in tracking contamination routes in the production line of pasteurized milk. Iranian J Vet Res Shiraz Uni.2010;11(3):1421-1429 https://doi.org/10.3168/jds.s0022-0302(01)70174-9
  22. Maurer J. PCR methods in foods. 1st ed. New York: Springer; 2006:69-90.https://doi.org/10.1007/0-387-31702-1
  23. PirondiniA, BonasU, Maestri E, VisioliG, MarmiroliM, Marmiroli N.Yield and amplificability of different DNA extraction procedures for traceability in the dairy food chain. Food Control.2010;21:663-668. https://doi.org/10.1016/j.foodcont.2009.10.004
  24. Marchand S, Vandriesche G, Coorevits A, Coudijzer K, De Jonghe V, Dewettinck K, De Vos P, Devreese B, Heyndrickx M, De Block J. Heterogeneity of heatresistant proteases from milk Pseudomonas species. Int J Food Microbiol. 2009;133:68-77. https://doi.org/10.1016/j.ijfoodmicro.2009.04.027
  25. Maeis T, Hameed M, Asmaa A. Isolation and Identification of protease producingAeromonas hydrophila. J Al-Nahrain Uni Sci.2014;17(1):143-147. https://doi.org/10.22401/jnus.17.1.19
  26. Melas DS, Papageorgiou DK,Mantis AI. Enumeration and confirmation of Aeromonas hydrophila, Aeromonas caviae, and Aeromonas sobria isolated from raw milk and other milk products in Northern Greece.J Food Prot.1999;62(5):463-6. https:// doi.org/ 10.4315/ 0362-028x-62.5.463
  27. Rasolofo EA,St-Gelais D, LaPointe G,Roy D. Molecular analysis of bacterial population structure and dynamics during cold storage of untreated and treated milk. Int J Food Microbiol. 2010;31(138):1-2. https://doi.org/10.1016/j.ijfoodmicro.2010.01.008
  28. MajeedK, EganA, Mac I. Production of exotoxins by Aeromonas spp at 5 C.J Appl Bacteriol.1990;69:332-337. https:// doi.org/10.1111/j. 1365-2672. 1990.tb01524.x
  29. Mateos D, Anguita J, Naharro G, Paniagua C. Influence of growth temperature on the production of extracellular virulence factors and pathogenicity of environmental andhuman strains of Aeromonas hydrophila. J Appl Bacteriol.1993;74:111-118. https://doi.org/ 10.1111/j .1365-2672.1993.tb03003.x
  30. Grassi M, Civera T,Turi R. Isolation of cytotoxic Aeromonas spp. from food. Vet Res Commu.2003;27:305-306. https://doi.org/ 10.1023/b:verc.0000014166.31914.1a
  31. Hantsis E, Halpern M. Chryseobacterium hai- fense sp. nov., a psychrotolerant bacterium isolated from raw milk. Int J Syst Evol Microbiol.2007;57:2344-2348. https//dOI: 10.1099/ijs.0.65115-0
  32. Gomashe AV, Dharmik PG, Zade BN. Proteolytic activity among psychrotrophic bacteria isolated from refrigerated raw milk. Inter J Innovations Bio Sci. 2012;2(3):100-103. https://doi.org/ 10.1111/j .1471-0307.2009.00542.x
  33. Fonseca CR, Bordin AM, Fernandes CE, Rodrigues CH, Corassin AG, Cruz CA, Oliveira F. Storage of refrigerated raw goat milk affecting the quality of whole milk powder. J Dairy Sci.2013;96:4716-4724. https://doi.org/10.3168/jds.2012-6120
  34. Lafarge V, Ogier JL, Girard MV, Leveau JY, Gruss A, DelacroixBA. Raw cow milk bacterial population shifts attributable to refrigeration. ApplEnviron Microbiol.2004;70:5644-5650. https://doi.org/10.1128/ aem. 70.9.5644-5650.2004
  35. Raats D, Offek M, Minz D, Halpern M. Molecular analysis of bacterialcommunities in raw cow milk and the impact of refrigeration on its structureand dynamics. Mol BiolEvol.2011;28:2731-2739. https://doi.org/10.1016/j.fm.2010.10.009
  36. MunschP,AlatossavaT. Phenotypic characterization of raw milk associated psychrotrophic bacteria. MicrRes.2005;161:334-346. https://doi.org/10.1016/j.micres.2005.12.004
  37. Barbano DM, Ma Y,Santos MV. Influence of raw milk quality on fluid milk shel life. JDairy Sci. 2006;89(E):15-19. https://doi.org/10.3168/jds.s0022-0302(06)72360-8
  38. Sadeq J.N,Fahed Kh.K,Hassan H.J.detection of Escherichia Coli hylAgene and Staphylococccus aureus Sea gene in raw milk buffaleosusing RT-PCR technique in AL-Qasisiyah province.Iraqi J Vet Sci. 2018;32(1):87-92. https://doi: 10.33899/ijvs. 2018. 153815
  39. Saleh Z.F, Al-Muhana B.M, Hamdan Kh, Jawad M.S, Klaif S.F. Isolation and identification of Salmonella typhimurium bacteria with detection of type-1 fimbriae coding gene by polymerase chain reaction (PCR) technique. Iraqi J Vet Sci.2019; 33(1): 195-199. https://doi:10.33899/ijvs.2019.162961

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