• Register
  • Login
  • العربیة

Iraqi Journal of Veterinary Sciences

  1. Home
  2. Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats

Current Issue

By Issue

By Subject

Keyword Index

Author Index

About Journal

FAQ

Peer Review Process

News

Aims and Scope

Editorial Board

Editorial Staff

Publication Ethics

Indexing and Abstracting

Related Links

Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats

    Authors

    • Sahar Mohammed Ibrahim 1
    • Thana’a M Ibrahim 2
    • Kareem Obayes Handool 3
    • Abubakar Adamu Abdul 4
    • jalila abu 5
    • Sabri Mohd Yusof 6
    • Mehdi Ibrahimmi 7
    • Loqman Yusof 8

    1 Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq

    2 مستشفى الصدر العام ، دائرة صحة الرصافة ،بغداد، العراق،

    3 Department of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, UPM

    4 Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, City Campus Complex, Usmanu Danfodiyo University, Sokoto Nigeria

    5 Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, UPM OF VETERINARY MEDICINE

    6 Department of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, UPM

    7 Department of Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, UPM VETERINARY MEDICINE

    8 Department of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, UPM TY OF VETERINARY MEDICINE

,

Document Type : Research Paper

10.33899/ijvs.2019.125832.1165
  • Article Information
  • References
  • Download
  • Export Citation
  • Statistics
  • Share

Abstract

In secondary fracture healing, callus proliferate, undergo hypertrophy and the extracellular matrix becomes calcified. This step to some extent, recapitulates the embryological bone development with a combination of cellular proliferation and differentiation, increasing cellular volume and matrix deposition. The causes of the chondrocytes volume increase in secondary bone healing are poorly known, but cell membrane transporters perhaps could be implicated. We hypothesize that NHE-1 and AE-2 are among plasma membrane transporters that have a role in cellular differentiation and regulation of endochondral ossification for secondary bone fracture healing. Study of closed tibia fracture healing in 2 groups of 25 of 8-weeks-old Sprague-Dawley rats were undertaken and histological evaluation were made at 5 different time points at 1, 2, 3, 4, and 6 weeks after induction of the fracture. Histological evaluation of proliferative and hypertrophic chondrocyte zone area showed a significant difference in week 1 compared to other weeks. Immunohistochemistry study revealed a significant high level of labeling intensity of NHE-1 at the first four weeks. While labeling intensity of AE-2 showed moderate reaction at 1 and 2 weeks, that increased and reached the highest level at 3 and 4 weeks. These results suggested that NHE-1 and AE-2 had role in the endochondral ossification of secondary bone healing.

Keywords

  • Histology
  • NHE-1
  • AE-2
  • Secondary bone healing

Main Subjects

  • General Surgery
  • XML
  • PDF 753.33 K
  • RIS
  • EndNote
  • Mendeley
  • BibTeX
  • APA
  • MLA
  • HARVARD
  • VANCOUVER
References
  1. Fayaz HC, Giannoudis PV, Vrahas MS, Moran C, Pape HC, Krettek C, Jupiter JB. The role of stem cells in fracture healing and nonunion. Int J Orthop. 2011;35(11):1587-1597. doi: 10.1007/s00264-011-1338-z
  2. Shapiro F. Bone development and its relation to fracture repair. The role of mesenchymal osteoblasts and surface osteoblasts. Eur Cell Mater. 2008;15:53-76. doi: 10.22203/eCM.v015a05
  3. Kim HJ, Liu X, Wang J, Chen X, Zhang H, Kim SH, Cui J, Li R, Zhang W, Kong Y, Zhang J, Shui W, Lamplot J, Rogers MR, Zhao C, Wang N, Rajan P, Tomal J, Statz J, Wu N, Luu HH, Haydon RC, He T. Wnt signaling in bone formation and its therapeutic potential for bone diseases. Ther Adv Musculoskel Dis. 2013;5(1):13-31. doi: 10.1177/1759720X12466608
  4. Sathyendra V, Darowish M. Basic science of bone healing. Hand clinics. 2013;29(4):473-81. doi: 10.1016/j.hcl.2013.08.002
  5. Bush PG, Pritchard M, Loqman MY, Damron TA, Hall AC. A key role for membrane transporter NKCC1 in mediating chondrocyte volume increase in the mammalian growth plate. J. Bone Miner. Res. 2010; 25(7):1594-603. doi: 10.1002/jbmr.47
  6. Loqman MY, Bush PG, Farquharson C, Hall AC. Suppression of mammalian bone growth by membrane transport inhibitors. J Cell Biochem. 2013;114(3):658-68. doi: 10.1002/jcb.24408
  7. Hoffmann EK, Lambert IH, Pedersen SF. Physiology of cell volume regulation in vertebrates. Phys Rev. 2009;89:193-277. doi: 10.1152/physrev.00037.2007
  8. Gerstenfeld LC, Alkhiary YM, Krall EA, Nicholls FH, Stapleton SN, Fitch JL, Bauer M, Kayal R, Graves DT, Jepsen KJ, Einhorn TA. Three-dimensional reconstruction of fracture callus morphogenesis. J Histochem Cytochem. 2006; 54(11):1215-28. doi: 10.1369/jhc.6A6959.2006
  9. Hankenso KD, Zmmerman G, Marcucio R. Biological perspectives of delayed fracture healing. Injury. 2014;45(2):S8-S15. doi: 10.1016/j.injury.2014.04.003
  10. Marsell R, Einhorn TA. The biology of fracture healing. Injury. 2011;42(6):551-5. doi: 10.1016/j.injury.2011.03.031
  11. Gibson JS, McCartney D, Sumpter J, Fairfax TPA, Milner PI, Edwards HL, Wilkins RJ. Rapid effects of hypoxia on H+ homeostasis in articular chondrocytes. Pflugers Arch Eur J Physiol. 2009;458:1085-1092. doi: 0.1007/s00424-009-0695-6
  12. Tattersall AL, Wilkins RJ. Modulation of Na+/H+ exchange isoforms NHE1 and NHE3 by insulin-like growth factor-1 in isolated bovine articular chondrocytes. J Orthop Surg Res. 2008;26:1428-1433. doi: 10.1002/jor.20617
  13. Tattersall AL, Meredith D, Furla P, Shen MR, Ellory JC, Wilkins RJ. Molecular and functional identification of the Na+/H+ exchange isoforms NHE1 and NHE3 in isolated bovine articular chondrocytes. Cell Physiol Biochem. 2003;13(4):215-222. doi: 10.1159/000072424
  14. Hannan KM, Little PJ. Mechanisms regulating the vascular smooth muscle Na+/H+ exchanger (NHE-1) in diabetes. Revue Canadienne de Biochimie et Biologie. 1998;76(5):751-759. doi: 10.1139/o98-093
  15. Yu L, Hales CA. Silencing of sodium-hydrogen exchanger 1 attenuates the proliferation, hypertrophy, and migration of pulmonary artery smooth muscle cells via E2F1. Am J Respir Cell Mol Biol. 2011;45(5):923-930. doi: 10.1165/rcmb.2011-0032OC
  16. Fliegel L. Regulation of the Na+/H+ exchanger in the healthy and diseased myocardium. Expert Opin Ther Targets. 2009; 13(1):55-68. doi: 10.1517/14728220802600707
  17. McKelvay D, Hollingshead KW. Veterinary anesthesia and analgesia. 3rd ed. New York: Mosby; 2004. 315-349 p.
  18. Greiff J. A method for the production of an undisplaced reproducible tibial fracture in the rat. Injury. 1978;9(4):278-81. doi: 10.1016/S0020-1383(77)80044-2
  19. Otto TE, Patka PM, Haarman HJTM. Closed fracture healing: A rat model. Eur Surg Res. 1995;27(4):277-84. doi: 10.1159/000129410
  20. Fedchenko N, Reifenrath J. Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue. Diagn Pathol. 2014;9(1):221. doi: 10.1186/s13000-014-0221-9
  21. Ramos J. Technical aspects of immunohistochemistry. Vet Pathol. 2005;42(4):405-426.
  22. Sfeir C, Ho L, Doll BA, Azari K, Hollinger JO. Bone regeneration and repair. In: Sfeir C, Ho L, Doll BA, Azari K, Hollinger JO. Fracture Repair. New Jersey: Humana Press; 2005. 21-44 p. doi: 10.1385/1-59259-863-3:021.
  23. Abed ER, Eesa MJ, Thanoon MG. Effects of platelets rich fibrin and bone marrow on the healing of distal radial fracture in local dogs: Comparative study. IJVS. 2019;33(2):419-25. doi: 10.33899/ijvs.2019.163169
  24. Amini S, Veilleux D, Villemure I. Three-dimensional in situ zonal morphology of viable growth plate chondrocytes: A confocal microscopy study. J Orthop Res. 2011;29(5):710-717. doi: 10.1002/jor.21294
  25. Marsh DR, Li G. The biology of fracture healing: Optimizing outcome. Br Med Bull. 1999;55(4):856-869. doi: 10.1258/0007142991902673
  26. Putney LK, Denker SP, Barber DL. The changing face of the Na+/H+ exchanger, NHE1: Structure, regulation, and cellular actions. Ann Rev Pharmacol. 2002;42:527-552. doi: 10.1146/annurev.pharmtox. 42.092001.143801
  27. Jansen ID, Mardones P, Lecanda F, de Vries TJ, Recalde S, Hoeben KA, Bronckers AL. AE-2a, b-Deficient mice exhibit osteopetrosis of long bones but not of calvaria. FASEB. 2009;23(10):3470-81. doi: 10.1096/fj.08-122598
  28. Masereel B, Pochet L, Laeckmann D. An overview of inhibitors of Na+/H+ exchanger. Eur J Med Chem. 2003;38(6):547-554. doi: 10.1016/S0223-5234(03)00100-4.
  29. Sagalovsky S. Bone remodeling: cellular-molecular biology and cytokine RANK- RANKL-Osteoprotegerin (OPG) system and growth factors. Crimean J Exp Clin Med. 2013;3(1-2):36-44. doi: 616-001.5:616.71-003.93.
    • Article View: 764
    • PDF Download: 966
Iraqi Journal of Veterinary Sciences
Volume 34, Issue 2
July 2020
Page 233-240
Files
  • XML
  • PDF 753.33 K
History
  • Received: 08 July 2019
  • Revised: 11 August 2019
  • Accept: 04 September 2019
  • Publish: 01 July 2020
Share
Export Citation
  • RIS
  • EndNote
  • Mendeley
  • BibTeX
  • APA
  • MLA
  • HARVARD
  • VANCOUVER
Statistics
  • Article View: 764
  • PDF Download: 966

APA

Ibrahim, S., Ibrahim, T. M., Handool, K., Abdul, A., abu, J., Yusof, S., Ibrahimmi, M., & Yusof, L. (2020). Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats. Iraqi Journal of Veterinary Sciences, 34(2), 233-240. doi: 10.33899/ijvs.2019.125832.1165

MLA

Sahar Mohammed Ibrahim; Thana’a M Ibrahim; Kareem Obayes Handool; Abubakar Adamu Abdul; jalila abu; Sabri Mohd Yusof; Mehdi Ibrahimmi; Loqman Yusof. "Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats". Iraqi Journal of Veterinary Sciences, 34, 2, 2020, 233-240. doi: 10.33899/ijvs.2019.125832.1165

HARVARD

Ibrahim, S., Ibrahim, T. M., Handool, K., Abdul, A., abu, J., Yusof, S., Ibrahimmi, M., Yusof, L. (2020). 'Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats', Iraqi Journal of Veterinary Sciences, 34(2), pp. 233-240. doi: 10.33899/ijvs.2019.125832.1165

VANCOUVER

Ibrahim, S., Ibrahim, T. M., Handool, K., Abdul, A., abu, J., Yusof, S., Ibrahimmi, M., Yusof, L. Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats. Iraqi Journal of Veterinary Sciences, 2020; 34(2): 233-240. doi: 10.33899/ijvs.2019.125832.1165

  • Home
  • About Journal
  • Editorial Board
  • Submit Manuscript
  • Contact Us
  • Glossary
  • Sitemap

News

  • IJVS in the first rank during Iraqi Science day 2023-01-30

 

© 2023, College of Veterinary Medicine, University of Mosul

Newsletter Subscription

Subscribe to the journal newsletter and receive the latest news and updates

© Journal Management System. Powered by ejournalplus