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Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 203-207
10.33899/ijvs.2019.125707.1134

Abstract

The use of antibiotics in inappropriate on food producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). Antibiotic resistance in animals and humans has become a global problem that needs attention and immediate management by using specific antibiotics that used for therapeutic the infected animals. The aim of this study was to isolate and detect E. coli producing ESBL. All E. coli from the surface of dairy cow rectal swabs in Sendang District, Tulungagung Regency, Indonesia using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The results of this study showed that all the samples were suspected of being E. coli, based on the morphological growth of colonies on the EMBA media. The isolates were identified by using the biochemical tests. All the samples were positive. In this study the double disc synergy test (DDST) method was using to confirm the ESBL. The antibiotics were used amoxicylyn-clavulanate, ceftazidime and cefotaxime for DDST. In additional ESBL confirmation test was used the Vitek-2 method. The presence of ESBL producing by E. coli isolated from rectal dairy swabs in tulungagung was 6% (3/50).
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  1. WHO. Critically important antimicrobials for human medicine. 5th ed. Geneva; WHO Press; 2016. 1-2 p.
  2. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: A clinical update. Clin Microbiol Rev. 2005;18(4):657-686. doi: 10.1128/CMR.18. 4.657-686.2005
  3. Price LB, Graham JP, Lackey LG, Roess A, Vailes R, Silbergeld E. Elevated risk of carrying gentamicin-resistant Escherichia coli among US Puoltry workers. Environ Health Perspect. 2007;115(12):1738-1742. doi: 10.1289/ehp.10191
  4. Guerra B, Fischer J, Helmuth R. An emerging public health problem: acquired aarbapenemase-producing microorganisms are present in food-producing animals, their environment, companion animals and wild birds. Vet Microbiol. 2014;171(3-4):290-297. doi: 10.1016/ j.vetmic.2014.02.001
  5. Hordijk J, Schoormans A, Kwakernaak M, Duim B, Broens E, Dierikx C, Dik M, Jaap AW. High prevalence of fecal carriage of extended spectrum ß-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs. Front Microbiol. 2013;4 :274. Doi: 10.3389/fmicb.2013.00242
  6. Sanders A, Amanda M. Vitek 2 compact-identification and susceptibility testing. USA: Standard Operating Procedure; 2018. 4-24 p.
  7. Safitri RD, Cicilia R, Bintari IG, Hermawan IP, Effendi MH, Ernawati R, Rahmahani J. Detection of encoding gene extended spectrum beta lactamase on Escherichia coli isolated from broiler chicken meat in traditional market Surabaya. Inter J Develop Res. 2017;07(11):17254-17357. ISSN : 2230-9926.
  8. Effendi MH, Harijani N, Yanestria SM, Hastutiek P. Identification of shiga toxin-producing Escherichia coli in raw milk samples from dairy cows in Surabaya, Indonesia. Philippine J Ved Med. 2018;55(SI):109-114.
  9. Effendi MH, Harijani N, Budiarto, Triningtya NP, Tyasningsih W, Plumeriastusi H. Prevalensi of pathogenic Escherichia coli isolated from subclinical mastitis in east Java province, Indonesia. Indian Vet J. 2019;96(03):22-25.
  10. CSLI. Performances standards for antimicrobial susceptibility testing. 26th ed. New York: Clinical and Laboratory Standards Institute; 2016. 1-34 p.
  11. Santos LL, Moura RA, Agilar-Ramires P, Castro AP, Lincopan N. Current status of extended-spectrum β-laktamase (ESBL)-producing Enterobacteriaceae in animals. 1st ed. USA: Formatex; 2013. 1600-1607 p.
  12. O'Brien TF. Emergence, spread, and environmental effect of antimicrobial resistance: how use of an antimicrobial anywhere can increase resistance to any antimicrobial anywhere else. Clin Infect Dis. 2002;3:78-84. doi: 10.1086/340244
  13. Ghatak S, Singha A, Sen A, Guha C, Ahuja A, Bhattacharjee U, Das S, Pradhan NR, Puro K, Jana C. Detection of New Delhi metallobeta-lactamase and extended spectrum beta-lactamase genes in Escherichia coli isolated from mastitic milk samples. Transbound Emerg Dis. 2013;60(5):385-9. doi: 10. 1111/tbed.12119
  14. Geser N, Stephan R, Kuhnert P, Zbinden R, Kaeppeli U, Cernela N, Haechler H. Fecal carriage of extended-spectrum β-laktamase-producing Enterobacteriaceae in swine and cattle at slaughter in Switzerland. J Food Protect. 2011;74(3):446-449. doi: 10.4315/0362-028X.JFP-10-372
  15. EFSA. Scientific opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamase in food and food-producing animals. EFSA J. 2011;9(8):1-95. doi: 10.2903/j.efsa.2011.2322
  16. Khosbayar T, Munguntsetseg B, Ochbadrakh B, Udval U, Batbaatar G, Wu J, Yong D. Plasmid analysis of ESBL producing gram negative bacilli in Mongolia. Mongolian J Hea Sci. 2013;10(1):90-100.
  17. Babypedmini S, Appalaraju B. Extended spectrum β–lactamases in urinary isolates of Escherichia coli and Klebsiella pneumonia prevalence and susceptibility pattern in a tertiary care hospital. Indian J Med Microbiol. 2004;22(3):172-4.
  18. Parasakthi N, Arrifin H, Kamarulzaman A, Ibrahim HSM, Adnan A, Choeng I. Consensus guidelines for the management of infections by ESBLproducing bacteria. Malaysian Soc Infect Dis Chemother. 2001;7:3.
  19. Sukmawinata E. Tingkat kejadian Escherichia coli penghasil extended spectrum β-lactamase di feses sapi di rumah potong hewan ruminansia kota Bogor [MSc thesis]. Bogor: Institut Pertanian Bogor; 2015. 1-185 p.
  20. Haenni M, Châtre P, Métayer V, Bour M, Signol E, Madec JY, Gay E. Comparative prevalence and characterization of ESBL producing Enterobacteriaceae in dominant versus subdominant enteric flora in veal calves at slaughterhouse, France. Vet Microbiol. 2014;171:321-327. doi: 10.1016/j.vetmic.2014.02.023
  21. Schmid A, Hörmansdorfer S, Messelhäusser U, Käsbohrer A, Sauter-Louis C, Mansfeld R. Prevalence of extended-spectrum β-lactamase-producing Escherichia coli on Bavarian dairy and beef cattle farms. Appl Environ Microbiol. 2013;79(9):3027-3032. doi: 10.1128/AEM.00204-13
  22. Odenthal S, Akinenden O, Usleber E. Extended-Spectrum β-Lactamase producing Enterobacteriaceae in bulk tank milk from German diary Farm. Inter J Food Micribiol. 2016;238:72-78. doi: 10.1016/j.ijfoodmicro.2016.08.036
  23. Kuntaman, Mertiasih NM, Hadi U. Multiresistance pattern of extended spectrum β-lactamase (ESBL)-Escherichia coli and Klebsiella pneumoniae strains. Folia Med Indones. 2006;42(1):40-46.
  24. Bramantono PH. The prevalence of extended spectrum betalactamase (ESBL) in third generation cephalosporin usage among sepsis patients in the department of internal medicine RSUD Dr Soetomo Surabaya. Folia Med Indones. 2012;49(4):244-251.
  25. Severin JA, Mertaniasih NM, Kuntaman K, Lestari ES, Purwanto M, Toom NL, Duerink DO, Hadi U, Belkum A, Verburg HA, Goessens WH. Molecular characterization of extended-spectrum β-lactamases in clinical Escherichia coli and Klebsiella pneumoniae isolates from Surabaya, Indonesia. J Antimicrob Chemother. 2010;65:465-469. doi:10.1093/jac/dkp471
  26. CLSI. Performance standards for antimicrobial susceptibility testing. 24th ed. Pennsylvania: Clinical Laboratory and Standard Institute; 2014. 1-25.
  27. Munoz-Davila MJ, Roig M, Yague G, Blazquez A, Salvador C, Segovia M. Comparative evaluation of Vitek-2 identification and sucepbility testing of urinary tract pathogens directly and isolated from chromogenic media. Eur J Clin Microbiol Infect Dis. 2013;32:773-780. doi: 10.1007/s10096-012-1806-4
  28. Spanu T, Sanguinetti M, Tumbarello M, D’Inzeo T, Fiori B, Posteraro B, Santangelo R, Cauda R, Fadda G. Evaluation of the new VITEK-2 Extended-Spectrum Beta-Lactamase (ESBL) test for rapid detection of ESBL production in Enterobacteriaceae isolates J Clin Microbiol. 2006;44(9):3257-3262. doi: 10.1128/JCM.00433-06

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