Iraqi Journal of Veterinary Sciences

Current Issue

By Issue

By Subject

Keyword Index

Author Index

About Journal

FAQ

Peer Review Process

News

Aims and Scope

Editorial Board

Editorial Staff

Publication Ethics

Indexing and Abstracting

Related Links

Influence of chitosan on hematological and histopathological changes in mice infected with Brucella melitensis immunized with Rev - 1 vaccine

    Authors

    1 Department of Pathology and Poultry Diseases, College of Veterinary Medicine, Al-Qasim Green University, Babylon, Iraq

    2 Veterinary Directorate, Ministry of Agriculture, Babylon, Iraq

,

Document Type : Research Paper

Abstract

This study aimed to assess the changes of blood cells constitutions and study the histopathological sections of liver and spleen in dual sexes mice infected with brucella melitensis and the role of chitosan supplement with and without Rev-1 vaccine in enhancing the inflammatory proses, two experiments were undertaken on 100 albino mice, aged 8-10 weeks for 60 days, first experiment done on 50 males and second one done on 50 females. Results of hematological analysis showed that there are significant increase at P3), red blood cells count RBC (× 106), hematocrit (HCT), mean corpuscular value (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), lymphocyte (LY%), granulocyte (GR) and lymphocytes m/mm3 (LYM) in males treated groups comparing with females, the highest values were in immunized infected mice with chitosan diet supplement compared to infected animals. The histopathological lesions recorded there is presence of chronic inflammatory reaction characterized by mononuclear cell infiltration with presence of granulomatous lesion in the liver of immunized animals, presence of mild to moderate lesions characterized by hyperplasia of lymphoid tissue in spleen and small granulomatous lesions in liver of immunized animals fed diet with chitosan supplement.

Keywords

Main Subjects

References
  1. Waqas A, Ke Z, Zheng L. Establishment of chronic infection: Brucella's stealth strategy. Front Cell Infect Microbiol. 2016;15(6):30. doi: 10.3389/fcimb.2016.00030.
  2. Franco MP, Mulder M, Gilman RH, Smits HL. Human brucellosis. Lancet Infect Dis. 2007;7(12):775-786.
  3. Singh BB, Dhand NK, Gill JP. Economic losses occurring due to brucellosis in Indian livestock populations. Prev Vet Med. 2015;119(3-4):211-215.
  4. Durward M, Radhakrishnan G, Harms J, Bareiss C, Magnani D, Splitter GA. Active evasion of CTL mediated killing and low quality responding CD8+T cells contribute to persistence of brucellosis. PLoS ONE. 2012;7:e34925.
  5. Bossi P. Bichat guidelines for the clinical management of brucellosis and bioterrorism-related brucellosis. Euro Surveill 2004;9:e15-e16.
  6. Scholz HC, Nöckler K, Göllner CG. “Brucella inopinata sp nov, isolated from a breast implant infection,”. Inter J Sys Evol Microbiol. 2010;60(4):801-808.
  7. Pappas G, Papadimitriou P, Christou L, Akritidis N. Future trends in human brucellosis treatment. Expert Opin Invest Drugs. 2006;15:1141-1149.
  8. De Figueiredo P, Thomas A, Ficht A, Carlos A, Rossetti A, Garry A. Pathogenesis and immunobiology of Brucellosis: Review of Brucella host interactions. Am J Pathol. 2015;185:1505-1517.
  9. Vitry MA, Delphine HMs, Michael D, Katrin H, Arnaud M, Frédéric L, Jean V, Carl De Tz, David P, Jean L, Eric M. Brucella melitensis invades murine erythrocytes during infection. Infect Immun. 2014;82(9):3927-3938.
  10. Pappas G, Kitsanou M, Christou L, Tsianos E. Immune thrombocytopenia attributed to brucellosis and other mechanisms of Brucella-induced thrombo-cytopenia. Am J Hematol. 2004;75:139-141.
  11. Grigoriou I, Giannakaki A, Pagoni M, Themistoklis D Karmiris C. Mitsouli, E. Nikiforakis. Extreme hematological abnormalities associated with human brucellosis: Report of two cases. Haema. 2003;6:93-96.
  12. Santosh K, Joonseok K, Hyerim K, Gupta MK, Dutta PK. A new chitosan thymine conjugates: Synthesis, characterization and biological activity. Inter J Biol Macromol. 2012;50:493-502.
  13. Vo TS, Kim JA, Ngo DH, Kong CS, Kim SK. Protective effect of chitosan oligosaccharides against FcɛRI-Mediated RBL-2H3 mast cell activation. Process Biochem. 2012;47:327-330.
  14. Wang TJ. Assessing the role of circulating, genetic, and imaging biomarkers in cardiovascular risk prediction. Circ. 2011;123:551-565.
  15. Chung MJ, Park JK, Park YI. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice. Int Immunopharmacol. 2012;12:453-459.
  16. Cobrado L, Silva A, Azevedo MM, Pina C, Rodrigues AG. In vivo antibiofilm effect of cerium, chitosan and hamamelitannin against usual agents of catheter-related bloodstream infections. J Antimicrob Chemother Pmid. 2013;68(1):126.
  17. Jayakumar R, Prabaharan M, Sudheesh PT, Nair SV, Tamura H. Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv. 2011;29:322-337.
  18. Qin YK, Xing RG, Liu S, Li KC, Meng XT, Li RF, Cui JH, Li B, Li PC. Novel thiosemicar- bazone chitosan derivatives: Preparation, characterization, and antifungal activity. Carbohydr Polym. 2012;87:2664-2670.
  19. Alkhafajy M.A.J. The role of chitosan and immunization with Brucella melitensis Rev1- vaccine in reducing the pathogenesis and oxidative stress induced by virulent Brucella melitensis in albino mice. {PhD thesis] Baghdad university College of Veterinary Medicine; 2017. 36-38p.
  20. Quinn PJ, Carter ME, Markey B, Carter GR. Clinical veterinary microbiology. 6th ed. London: Mosby; 2004. 261-267 p.
  21. Shakir SA. Effect of hypercholesterolemia, chitosan and whole sonicated E. coli Ags on immune response and pathological changes in mice infected with E. coli (O:127) isolated from children suffering from diarrhea. [MSc thesis] Baghdad: College of Veterinary Medicine; 2012. 30 p.
  22. Milles A, Misra S, Irwin J. The estimation of bactericidal power of the blood. J Hyg. 1938;38:732.
  23. Luna LG. Manual of histological staining of force institute of pathology. 3rd ed. New York: McGraw hill book; 1968. 134-135 p.
  24. Al-Zubaidy K, Al-Falahy M. Principles and procedures of statistics designs and experimental designs. Ministry of Higher Education and Scientific Research, University of Duhok, College of Agriculture, Iraq; 2018. 239-267 p.
  25. SAS. SAS Users Guide: Statistical version 6.12. North Carolina: SAS institute Inc; 2012. 722 p.
  26. Kumar A, Yadav SK. Assessment of hematological bio markers during vaccination and challenge of Brucella melitensis in Goats. Int J Vaccines Vaccin. 2017;4(2):1-6.
  27. Tsevelma N, Odgerel O, Dariimaa D, Batkhuu J. Anti-Brucella activity of Caryopteris mongolica Bunge root extract against Brucella melitensis infection in mice. Complement Altern Med. 2018;18:144.
  28. Copin R, Vitry M, Hanot D, Machelart A, De C, Vanderwinden J, Magez S, Akira S, Ryffel B, Carlier Y, Letesson J, Muraille E. In situ microscopy analysis reveals local innate immune response developed around Brucella infected cells in resistant and susceptible mice. PLoS Pathog. 2012;8:1-5.
  29. Suzuki K. Okawa Y, Hashimoto K. Protecting effect of chitin and chitosan on experimentally induced murine candidiasis. Microbiol Immunol. 1984;8(28):903-912.
  30. Aleksandra B, Anna W, Bogumila P, Alojzy R, Lukasz L. Antibacterial and antifungal activity of chitosan. ISAH. 2005;2:1-3.
  31. Abdulkadir K, Tekin T, Mehmet T, Gülali A, İsmail N, Fırat Z, Hayrettin A. Erythrocytes parameters in the course of Brucellosis. Abant Med. 2013;39(2):36-39.
  32. Droge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002;82:47-95.
  33. Loguercio C, Federico A. Oxidative stress in viral and alcoholic hepatitis. Free Radic Biol Med. 2003;34(1):1-10.
  34. Pierce JD, Cackler AB, Arnett MG. Why should you care about free radicals. RN. 2004;67(1):38-42.
  35. Alwan MJ. Study of some aspects of nocardiosis in animals in Iraq [PhD dissertation]. Baghdad: College of Veterinary Medicine, University of Baghdad; 1996; 98 p.
  36. William C, Steven C, Olsen C, Jack W, Betsy J, Mitchell V, Palmer E, Jensen M, Halling A. An overview of Diagnostic characterization of a feral swine herd enzootically infected with Brucella. J Vet Diagn Invest. 2015;19:227-237.
  37. Stiff A, Trikha P, Mundy B, McMichael E, Mace TA, Benner B, Kendra K, Campbell A, Gautam S, Abood D. Nitric oxide production by myeloid-derived suppressor cells plays a role in impairing Fc receptor-mediated natural killer cell function. Clin. Cancer Res. 2018;24:1891-1904.
  38. Smith A, Perelman M, Hinchcliffe M. Chitosan: A promising safe and immune-enhancing adjuvant for intranasal vaccines. 2014;10(3):797-807.
  39. Zaharoff DA, Rogers CJ, Hance KW, Schlom J, Greiner JW. Chitosan solution enhances both humoral and cell-mediated immune responses to subcutaneous vaccination. Vaccine. 2007;25(11):2085-94.
Iraqi Journal of Veterinary Sciences
Volume 34, Issue 1
January 2020
Page 23-29
Files
History
  • Received: 25 January 2019
  • Revised: 02 May 2019
  • Accepted: 12 May 2019
  • Published: 01 January 2020
Share
Export Citation
Statistics