Protective effect of aqueous extract of Alhagi maurorum in spermatogenesis and antioxidant status of adult rats exposed to carbon tetrachloride
Iraqi Journal of Veterinary Sciences,
2019, Volume 33, Issue 1, Pages 1-7
AbstractThis Study aimed to investigate the efficiency of aqueous extraction of Alhagi maurorum leaves against oxidative stress induced by Carbon tetrachloride (CCl4) on spermatogenesis and the level of glutathione, superoxide dismutase, malondialdehyde in adult rats. Plant Leaf's dried and then extracted. Experiment included 24 male rats divided into 4 groups 6 subjects in each group. groups treated orally for 30 days as following: first was control group which administered with 1ml of physiological saline 0.9%, second group administered once with CCl4 3ml/Kg, third and fourth groups administered with aqueous extract 300 mg/kg and aqueous extract together with CCl4 respectively. The results showed that CCl4 caused a significant decrease in sperm count, sperm vitality, normality, glutathione (GSH) and superoxide dismutase (SOD), significant increase in sperm mortality, abnormality and malondialdehyde (MDA) compared with control group. While aqueous extract treatment caused no significant difference in compare to control group. Groups treated with aqueous extract together with CCl4 showed a significant increase in sperm count, vitality, normality and GSH and decreasing in mortality, abnormality and MDA in compare to CCl4 group. It could be concluded that the aqueous extract of Alhagi maurorum have a positive effect on male reproduction and antioxidants in rats exposed to oxidative stress.
1- Zegers-Hochschild F, Adamson, GD, deMouzon J, Ishihara O, Mansour R, Nygren, K. International committee for monitoring assisted reproductive technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology. Fert Stert. 2009;92(5):1520-4.
2- Davies MJ, Moore VM, Willson KJ, Van Essen P, Priest K, Scott H. Reproductive technologies and the risk of birth defects. N Engl J Med. 2012;366(19):1803-13.
Agarwal A, Virk G, Ong C, Plessis S. Effect of oxidative stress on male reproduction. World J Mens Health. 2014;32(1):1–17.
3- Gharagozloo P, Aitken RJ. The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Hum Reprod. 2012;26:1628-40.
Aitken R, Roman S. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longevity. 2008;1(1):15-24.
Misro MM, Choudhury L, Upreti K, Gautam D, Chaki SP, Mahajan A S, Babbar R. Use of hydrogen peroxide to assess the sperm susceptibility to oxidative stress in subjects presenting a normal semen profile. Inter J Androl. 2004;27:82-87.
Neamah NF. A pharmacological evaluation of aqueous extract of Alhagi maurorum. Glob JPharmacol. 2012;6(1):41-6.
Loizzo M, Rashed K, Said A, Bonesi M, Menichini F, Tundis R. Antiproliferative and antioxidant properties of Alhagi maurorum Boiss (Leguminosae) aerial parts. Indus Crop Prod. 2014;53:289-295.
Muhammad G, Hussain MA, Anwar F, Ashraf M, Gilani AH. Alhagi: a plant genus rich in bioactives for pharmaceuticals. Phytother Res. 2015;29(1):1-13.
Al-Snafi A. Alhagi maurorum as a potential medicinal herb: an overview. Inter J Pharm Rev Res. 2015;5:130-136.
Sheweita SA, Mashaly S, Newairy AA. Changes in oxidative stress and antioxidant enzyme activities in streptozotocin-induced diabetes mellitus in rats: Role of Alhagi maurorum Extracts. Oxid Med Cell Longevity. 2016;1:1-8.
Rehman JU, Aktar N, Khan MY, Ahmad K, Ahmad M, Sultana S. Phytochemical screening and hepatoprotective effect of Alhagi maurorum boiss (Leguminosae) against paracetamol-induced hepatotoxicity in rabbits. Trop J Pharm Res. 2015;14:1029–34.
Mauricio J, Penarrietaabc J, Alvaradoa A, Akessonb B, Bergenstahlc B. Separation of phenolic and flavonoid compounds from foods by reversed-phase high performance liquid chromatograph. Bolivian J Chem. 2007;24(1):1-4.
Khanam Z, Sam K, Mohd N, Ching C, Haq Bhat I. Analyses and DNA cleavage activity of Malaysian Averrhoa carambola L. fruit extracts. J Kin Saud Uni Sci. 2015;27:331-337.
Bankroft JD, Stevens A. Theory and practice histological technique. 5th ed. London: Churchill Livingston; 1982. P.25-28.
Pant N, Murthy RC, Srivastava SP. Male reproductive toxicity of sodium arsenate in mice. Hum Exp Toxicol. 2004;23:399-403.
Wells ME, Awa OA. New technique for assessing acrosomal characteristic of spermatozoa. J. Diary Sci. 1970;53:227.
Hall JA. Effect of transport on blood selenium and glutathione status in feeder lambs. J Anim Sci. 2014;92:4115-4122.
Halliwell B, Chirico S. Lipid peroxidation: its mechanism, measurement and significance. Am J Clin Nutr. 1993;57:715-24.
Al-Mohammed NT, Al-Rawi KM, Younis MA, Al-Morani WK. Principles of statistics. Mosul: Mosul University Press; 1986. P.85-94.
Halliwell B. Oxidative stress and neurodegeneration: where are we now? J Neurochem. 2006;58:1634-36.
Wang X, Sharma RK, Sikka SC, Thomas AJ, Falcone T, Agarwal A. Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility. Fert Stert. 2003;80:531-535.
Sikka S. Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl. 2004;25, 5-18.
Lotito SB, Frei B. Consumption of flavonoid-rich foods and increased plasma antioxidant capacity in humans cause consequence or epiphenomenon. Free Radic Biol Med. 2006;41(12):1727-46.
Asadi N, Bahmani M, Kheradmand A, Rafieian-Kopaei M. The impact of oxidative stress on testicular function and the role of antioxidants in improving it: A review. J Clin Dig Res. 2017;11(5):1-5.
Laghari AH, Memon A, Memon S, Nelofar A, Khan KM, Yasmin A. Determination of free phenolic acids and antioxidant capacity of methanolic extracts obtained from leaves and flowers of camel thorn (Alhagi maurorum). Nat Prod Res. 2012;26(2):173-6.
Ahmad N, Bibi Y, Saboon I, Zahara K, Idrees S, Nadia K, Bashir T, Tabassum S, Mudrikah I. Traditional uses and pharmacological properties of Alhagi maurorum: A review. Asian Pacific J Trop Dis. 2015;5(11):856-861.
Peterson J, Lagiou P, Samoli E, Lagiou A, Katsouyanni K, La Vecchia C, Dwyer J, Trichopoulos D. Flavonoid intake and breast cancer risk: a case-control study in Greece. Br J Cancer. 2003;89:1255-1259.
Romeo C, Antonuccio P, Esposito M, Marini H, Impellizzeri P, Turiaco N. Hydrophilic vitamin e like antioxidant reduces testicular ischemia perfusion injury. Urol Res. 2004;32:367-71.
Szabo C, Ischiropoulus H, Radi R. Peroxynitrite biochemistry, pathophysiology and development of therapeutics. Nat Rev. 2007;6:662-79.
Bahmani M, Mirhoseini M, Shirzad H, Sedighi M, Shahinfard N, Rafieian M. A review on promising natural agents effective on hyperlipidemia. J Evid Based Compl Altern Med. 2015;20(3):228-38.
- Article View: 4,686
- PDF Download: 1,476