TY - JOUR ID - 175272 TI - Molecular identification of virulence genes of Pseudomonas aeruginosa isolated from fish (Cyprinus carpio) in Mosul city JO - Iraqi Journal of Veterinary Sciences JA - IJVS LA - en SN - 1607-3894 AU - Altaee, Asmaa J. AU - Aldabbagh, Sumaya Y. AD - Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq Y1 - 2022 PY - 2022 VL - 36 IS - 4 SP - 953 EP - 958 KW - Cyprinus carpio KW - P. aeruginosa KW - Virulence genes DO - 10.33899/ijvs.2022.132660.2119 N2 - Depending on the nature of the fish's environment, they are susceptible to many pathogens, including bacterial causes, so the goals of the current study are isolation and molecular identification of Pseudomonas aeruginosa with its prevalence rate and detected virulence genes from fresh common carp fish. The swabs were taken from the gills, skin, intestine and muscles of 75 fish samples from variable localities from Mosul city during the period September to December in the year 2021. The prevalence percentage of bacteria was 26.66% which was confirmed by traditional microbiological tests which included (phenotype culture, microscopically features and API-test) and molecular identification. The isolates formed 42.5, 37.5, 15, 5% from gills, skin, intestine and muscles, respectively. The molecular results of forty isolates determine that Pseudomonas aeruginosa have rpoB 100%, and virulence genes oprL, toxA, and algD, which are express the outer membrane protein, exotoxin A and alginate respectively occur as 97.5% for the oprL gene and 100% of both toxA and algD genes. UR - https://www.vetmedmosul.com/article_175272.html L1 - https://www.vetmedmosul.com/article_175272_36454de1397eab1e338e549ea4e3e3db.pdf ER -