TY - JOUR ID - 172941 TI - Infections and molecular characterization of anisakid nematodes from two species of marine fish northwest Arabian gulf JO - Iraqi Journal of Veterinary Sciences JA - IJVS LA - en SN - 1607-3894 AU - Bannai, Majid A. AU - Jori, Muna M. AD - Department of Marine Vertebrate, Marine Science Center, University of Basrah, Basrah, Iraq AD - Departments of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq Y1 - 2022 PY - 2022 VL - 36 IS - 2 SP - 489 EP - 497 KW - Anisakid KW - Arabian Gulf KW - Molecular characterization KW - E. coioides KW - E. diacanthus DO - 10.33899/ijvs.2021.130613.1851 N2 - The present study provides new insight into valuable information on the diverse structure of the anisakid population, discusses the limited species richness, and also discusses the relationship with other closely diversity-related taxa in NCBI databases in the Epinephelus diacanthus and Epinephelus coioides fish. The fishing area consists of various locations in the Arabian Gulf. A total of 69 E.coioides and E. diacanthus were examined, (n= 48) were infected. Larval stages (n=1,119). Isolated larvae were encysted within the mesenteries peritoneum and viscera of fish organs, with a prevalence of 81.25% of infection and 59.459 % in the E. diacanthus and E. coioides respectively. Molecular analysis was carried out on thirty individuals of nematode parasites who have examined the morphology and showed some appearance differences, by amplifying internal transcribed spacers ITS and ITS-1 of nuclear rDNA (rDNA) by PCR using the primer sets NC5/NC2 and SS1/NC13R of DNA products. Evolutionary analyses were conducted in MEGA X. based on the identity percentage in the GenBank database showed that they belong to anisakid nematodes, in particular, they belong to nine distinct taxa within the Hysterothylacium spp. The presence of the same species individuals in one host may be the cause of these genetic variations at the species level, and that's what the current study has recorded. It has been found that there is an overlap in the order of nitrogen bases between the same species, and this occurs through the fertilization process, while the rest is clean or have only a few parasites.  UR - https://www.vetmedmosul.com/article_172941.html L1 - https://www.vetmedmosul.com/article_172941_59d935bac8a5e222bf25e8ecbcacebf2.pdf ER -