TY - JOUR ID - 168441 TI - Effect of ultrasound on protoscoleces of Echinococcus granulosus in vitro and in vivo JO - Iraqi Journal of Veterinary Sciences JA - IJVS LA - en SN - 1607-3894 AU - Ali, Asmaa A. AU - Ramadhan, Baraa B. AD - Department of Biology, College of Pure Sciences, University of Mosul, Mosul, Iraq Y1 - 2021 PY - 2021 VL - 35 IS - Supplement I-III SP - 1 EP - 5 KW - Echinococcus granulosus KW - ultrasound KW - delayed-type hypersensitivity DO - 10.33899/ijvs.2021.126906.1410 N2 - The current study was designed to investigate the effect of ultrasound on the protoscoleces of Echinococcus granulosus by enforcing steady numeral of frequencies during a certain interval (20000 pulse/s) (1.8 w/cm2), using exposure time 30,20,15,12,10 and 5s, individually and respectively. Consequently, six albino mice groups were immunized against cystic echinococcosis, which injected with exposed protoscoleces, to acquire specific cell-mediated immunity, called delayed type-hypersensitivity (DTH) which assessment by measurement the foot pad density. The results displayed significant excess (P≤ 0.001) of DTH by increase of foot pad thickness in injected groups. The results showed maximum thickness of 1.54 mm, 1.4mm, 0.9 mm. after 3h, 24h, and 48h post - injection, respectively, that compared with the thickness of control group 1.072, 0.638, 0.328 mm, respectively, during five months of experiment. The present research exhibited the action of ultrasonication technique on the viability of Echinococcus granulosus protoscoleces in vitro. Conclusion, Ultrasound frequencies used in the present study could be have consequential impact on the cellular immunity in albino mice. UR - https://www.vetmedmosul.com/article_168441.html L1 - https://www.vetmedmosul.com/article_168441_415faf58f769e44f6cd500904749f5f3.pdf ER -