TY - JOUR ID - 164543 TI - Molecular characterization of fertile hydatid cysts from the liver of the sheep and cows and associated environmental influence factors JO - Iraqi Journal of Veterinary Sciences JA - IJVS LA - en SN - 1607-3894 AU - Abdulla, Renas G. AU - Mageed, Sarmad N. AU - Obed, Chawarwan E. AU - Jumaa, Jamil A. AD - Department of Biology, Faculty of Science and Health, Koya University, Erbil, Iraq AD - Department of Medical Microbiology, Faculty of Science and Health, Koya University, Koya City, Erbil, Iraq. AD - Department of Biology, Faculty of Science and Health, Koya University, Koya, Iraq AD - Department of Chemistry, Faculty of Science and Health, Koya University, Erbil, Iraq Y1 - 2020 PY - 2020 VL - 34 IS - 2 SP - 321 EP - 327 KW - Hydatid cysts KW - Cytochrome C oxidase KW - Sheep strain KW - Phylogenetics KW - Cyclic-voltammetry DO - 10.33899/ijvs.2019.126036.1213 N2 - The aim of the study is characterizing of hydatid cysts that have been isolated from sheep and cow liver fertile hydatid cysts using mitochondrial cytochrome c oxidase subunit 1(cox1) gene. DNA samples of nineteen sheep and one cow were extracted from protoscoleces and germinal layers of the parasites in Koya city-Erbil, Iraq, using specific extraction procedures. Mitochondrial cox1 gene region was amplified by polymerase chain reaction (PCR), and the expected gene sizes were confirmed by gel electrophoresis. All DNA isolates were then sequenced. Nucleotide sequence alignments were then performed to verify the sequenced isolated according to the database, which showed that all samples were belonging to the (G1) sheep strain. Phylogenetic analysis was also carried out for the sequenced isolated to find out the highest similarities with closest organisms to E. granulosus’ conserved gene and to reveal sharing common ancestor, which has been confirmed. Electrocatalytic reduction of DNA where detected through applying cyclic-voltammetry technique, which referred to the environmentally strong protection features of these strains against any effects of external factors, such as heavy metals and has revealed the secret behind the potent preservation of the DNA structure of this parasite from being affect by mutations, or alterations, along the different lineages over a long period of time. UR - https://www.vetmedmosul.com/article_164543.html L1 - https://www.vetmedmosul.com/article_164543_f779754feb56a4f0f260a1647234a435.pdf ER -