TY - JOUR ID - 163566 TI - Detection of methicillin-resistant <i>Staphylococcus aureus</i> in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques JO - Iraqi Journal of Veterinary Sciences JA - IJVS LA - en SN - 1607-3894 AU - Assafi, Mahde S. AU - Hado, Hishiyar A. AU - Abdulrahman, Ibtessam S. AD - Department of Biology, School of Sciences, University of Duhok, Duhok, Iraq AD - Department of Biology, Faculty of Sciences, University of Zakho, Zakho, Duhock, Iraq AD - Department of Pharmaceutic, College of Pharmacy, University of Duhock, Duhok, Iraq Y1 - 2020 PY - 2020 VL - 34 IS - 1 SP - 15 EP - 22 KW - MRSA KW - <i>S. aureus</i> KW - mecA gene KW - Broilers KW - Farm workers DO - 10.33899/ijvs.2019.125757.1145 N2 - Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) has become a global public health concern. The purpose of this study was to investigate the prevalence rates of MRSA infection amongst broiler chickens and broilers farm workers. The total samples used in this study were 306. Cloacal swab samples from 231 broilers and nasal swab samples from 75 broilers farm workers were collected from five farms in Duhok city, Iraq. Isolation and identification of MRSA isolates were carried out and the antibiotic susceptibility were screened. Molecular characterization of all isolates was performed by using polymerase chain reaction (PCR) technique to detect the mecA gene.S. aureus was detected among 84% (63/75) of the farms workers samples and among 84.8% (196/231) of the broiler's samples. The S. aureus isolated from farm workers and broilers appeared resistant to oxacillin 28.6% (18/63), and 32.1% (63/196), respectively. MRSA colonization in farm workers and broilers was 24% (18/75) and 27.3% (63/231) respectively. The S. aureus isolates showed the most resistant to chloramphenicol and the least resistant to vancomycin. The results of the PCR assays revealed that 85.7% (12/14) of S. aureus isolates from farm workers and 44.4% (16/36) of S. aureus isolates from broilers were positive for the mecA gene. The direct handling of broilers by farm workers plays the important role for transport the MRSA isolates from broilers to broilers farm workers. UR - https://www.vetmedmosul.com/article_163566.html L1 - https://www.vetmedmosul.com/article_163566_e35fc5256eadb309c771dcebe9015acd.pdf ER -