Keywords : FSH

Effect of aqueous rosemary extract on some sexual hormones in male rats with high thyroxine level

Fouad Z. Hamza; Nabeel M. Al-Sharafi; Sumayah F. Kasim

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 2, Pages 369-373
DOI: 10.33899/ijvs.2020.126872.1404

The main purpose of this research is to evaluate the high level of thyroxine affects on each of the luteinizing hormone (LH), testosterone, follicle-stimulating hormone (FSH) of rats and to estimate the potential effect of the administration of aqueous rosemary extract and propylthiouracil against testicular toxicity induced by levothyroxine in male rats. Negative control group rats were treated with distilled water. Three groups of male rats were treated subcutaneously with 0.5 mg/kg levothyroxine for 12 days: Since day 13th to the 24th day of the experiment first group was regarded as a positive control group that received distilled water, the second group was given propylthiouracil at a dose level of 10 mg/kg b.wt, and the third was given aqueous extracts of rosemary 10 mg/kg b.wt. The results revealed that treatment with aqueous extract of rosemary lead to a significant decrease in the levels of serum testosterone while a highly significant decrease in testosterone, FSH, and LH levels in serum revealed after treatment with propylthiouracil. Thus, it can be concluded that the effect of propylthiouracil and rosemary aqueous extract resulted in dialectical hormonal results in which the favor was to propylthiouracil.

Immunohistochemical expression levels of anterior pituitary LH, FSH and testicular testosterone of Allium sativum extract and thymoquinone in male treated rats

N.F. Hassan

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 49-55
DOI: 10.33899/ijvs.2018.153793

The present study was conducted to determine the immunohistochemical expression levels of Luteinizing Hormone and Follicle Stimulating Hormone in the anterior pituitary gland and testicular testosterone of male rats treated with Allium sativum extract and Thymoquinone. Forty mature Waster male rats Rattus norvegicus, aged 60 days and weighed 140-150 g, were randomly divided into control and three treatment groups (10 rats each). Control group males were orally supplemented with 500 µL of normal saline daily. The treated groups of males were orally supplemented with TQ suspension, 50 mg/ kg, B.Wt (TQ group), Allium sativum aqueous extract, 50 mg/ kg bw (A group) and their combination (TQA group). At the end of experiment, all male rats were sacrificed and pituitaries and testes were dissected and fixed in neutral formalin buffer 10% for immunohistochemical examination of pituitary LH and FSH and testicular testosterone. The results demonstrated high immunohistochemical density in treated male’s pituitary tissues for both LH and FSH compared with the control group. In comparison with control, testicular tissues of treated group males showed stronger immune reaction for T especially around Lydig’s and Sertoli cells. It can be concluded that TQ and Allium sativum have potent improving effects on reproductive hormones production and could be increase the male fertility.