Keywords : PCR technique


Molecular detection of mecA gene in methicillin-resistant Staphylococcus aureus isolated from dairy mastitis in Nineveh governorate, Iraq

Omar H. Sheet

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 4, Pages 939-943
DOI: 10.33899/ijvs.2022.132643.2115

Staphylococcus (S.) aureus is universally the leading aetiologic cause of dairy mastitis. Additionally, methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogenic bacterium in veterinary medicine and public health. Sixty-six cattleʼs milk samples were collected randomly from different areas of the Nineveh Province from November 2018 to February 2020. In this study, the classical and molecular biology methods had used to identify the MRSA and detect the target genes. The results revealed that S. aureus was isolated and identified based on classical methods such as catalase, clumping factors, and coagulase test. In addition, the nuc gene was detected in all the positive S. aureus isolates 23 (34.8%), while the mecA gene was detected in 12 (52.2%) MRSA isolates by using polymerase chain reaction (PCR) assay. The present work emerged that the results of classical methods and the PCR technique were similar. MRSA is regarded as a significant causative agent of various types of bovine mastitis in Iraq, and it can to resist all types of beta-lactams. MRSA isolated from different regions in Mosul city. PCR assay is a powerful method for detecting the different genes based on the target sequence of the specific gene.

Detection of the nuc gene in Staphylococcus aureus isolated from swamps and ponds in Mosul city by using PCR techniques

Omar H. Sheet; Reem A. Talat; Ibtihal I. Kanaan; Ashwaq A. Najem; Ali S. Saeed Alchalabi

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 3, Pages 821-824
DOI: 10.33899/ijvs.2022.173276.2069

In most developing countries, rainwater is considered a significant water source for drinking, washing, bathing, and cooking. On the other hand, this water is the medium for transporting microorganisms such as bacteria, viruses, parasites, and fungi to humans and animals. Most domestic and wild animals drink this kind of waterborne illness that leads to various types of diseases, which causes enormous economic losses. The current study was aimed to isolate Staphylococcus (S.) aureus from the swamps and ponds in various areas, including (Qawseat, Kukagle, Besan, Al-Arabi, and Al-Shlalat) that surrounding the Mosul city during the rainfall season. One hundred rainwater samples were collected from swamps and ponds in various Mosul city areas. The classical method and polymerase chain reaction (PCR) technique had used to identify S. aureus isolates. The present study showed that the prevalence rate of S. aureus isolated from swamps and ponds was 12% (12/100) based on the classical and PCR methods used. All the positive S. aureus isolates possess the specific-species nuc gene. In addition, the results of the classical methods are similar to the results of the PCR technique. The present study concludes that the water of swamps and ponds is formed by rainwater exposed to contamination by S. aureus, which posed in the ground and is not fit for the drinking of animals and humans.