Keywords : S. aureus

Detection of the nuc gene in Staphylococcus aureus isolated from swamps and ponds in Mosul city by using PCR techniques

Omar H. Sheet; Reem A. Talat; Ibtihal I. Kanaan; Ashwaq A. Najem; Ali S. Saeed Alchalabi

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 3, Pages 821-824
DOI: 10.33899/ijvs.2022.173276.2069

In most developing countries, rainwater is considered a significant water source for drinking, washing, bathing, and cooking. On the other hand, this water is the medium for transporting microorganisms such as bacteria, viruses, parasites, and fungi to humans and animals. Most domestic and wild animals drink this kind of waterborne illness that leads to various types of diseases, which causes enormous economic losses. The current study was aimed to isolate Staphylococcus (S.) aureus from the swamps and ponds in various areas, including (Qawseat, Kukagle, Besan, Al-Arabi, and Al-Shlalat) that surrounding the Mosul city during the rainfall season. One hundred rainwater samples were collected from swamps and ponds in various Mosul city areas. The classical method and polymerase chain reaction (PCR) technique had used to identify S. aureus isolates. The present study showed that the prevalence rate of S. aureus isolated from swamps and ponds was 12% (12/100) based on the classical and PCR methods used. All the positive S. aureus isolates possess the specific-species nuc gene. In addition, the results of the classical methods are similar to the results of the PCR technique. The present study concludes that the water of swamps and ponds is formed by rainwater exposed to contamination by S. aureus, which posed in the ground and is not fit for the drinking of animals and humans. 

Isolation and molecular detection of enterotoxigenic Staphylococcus aureus from raw milk of cows

Dunya D. Taher; Safana A. Yassin; Mariam H. Abdulkareem

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue Supplement I-III, Pages 137-141
DOI: 10.33899/ijvs.2021.131957.2030

Staphylococcus aureus is a microbe associated with human’s food poisoning. It is caused by isolates producing different heat stable enterotoxins which act as one of the most spread worldwide gastroenteritis causes. The goal of the current study was to assess the incidence of S. aureus (enterotoxigenic) in the raw milk samples of cows which were collected from various places of Baghdad city. Isolation and identification of S. aureus were done by conventional laboratory method and the diagnoses were confirmed by using molecular method RT-PCR to detect the specific gene of S. aureus nuc gene. In addition, we investigate the occurrence of some of Staphylococcal enterotoxins genes such as SEA, SEB, SEC, SED, and SEE. We found that 12/50 (24%) of the isolates were S. aureus and these isolates carried one or more of the enterotoxin genes such as enterotoxin type SEC 12/12 (100) followed by enterotoxin type SEA 7/12 (58.33%) and only 1/12 (8.33%) was type SED, while type SEB and SEE were negative 0%. Some of the isolates have genes that coded for two enterotoxins patterns such as SEA and SEC 7/12 (58.33%) and SEC and SED 1/12 (8.33%). Fewer of the isolates expressed only one genotype of enterotoxin gene like SEC 4/12 (33.3%). This study has proven that there was a high chance of occurrence for the enterotoxigenic S. aureus at the raw milk of cows in Baghdad city.