Keywords : Alpha lipoic acid

Assessment of the analgesic effect of Alpha-lipoic acid by three acute pain models

Marwa R. Rehab; Ahmed S. Naser

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 3, Pages 803-807
DOI: 10.33899/ijvs.2022.132184.2062

Alpha-lipoic acids are Known as a good analgesic in neuropathic pain, especially in diabetic patients. This research aimed to assess the analgesic activity of ALA by three acute pain models using broiler chicks. We used electrical stimulation, hot water test, and formalin test to elicit acute pain. The up and down method was used to calculate the median effect of the analgesic dose. The ED50 of ALA was 45.18 and 74.56 mg/kg intraperitoneally by electrical stimulation and hot water test, respectively. We demonstrated that the peak of analgesic effect was after one hour by using different doses and different times. ALA at 0, 75, 150, 300 mg/kg intraperitoneally produces a dose-dependent analgesic effect by formalin test. In conclusion: ALA induced analgesic activity, probably by closing voltage-gated calcium and or voltage-gated sodium channels. These outcomes show that therapeutic doses of ALA can affect pain and may mask or reduce nociception induced by acute pain models.

Role of alpha lipoic acid in protecting testes of adult rats from lead toxicity

Bara N. Al-Okaily; Haidar F. Murad

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 2, Pages 305-312
DOI: 10.33899/ijvs.2020.126814.1386

The current study was conducted to investigate the role of alpha lipoic acid (ALA) against testicular toxicity- induced by lead acetate (PbAc) in rats. Four groups of adult Wistar albino rats (8 for each) were intubated daily for 56 days as follows; control (C)received dislled water; lead acetate at dose of 5mg/kg b.w (T1); ALA at dose of 60mg/kg b.w (T2) and and group T3 received both of PbAc + ALA at the same doses of above. Blood samples were collected at 0, 28 and 56 day of the experiment, then the sera were collected for determination of testosterone(T) and follicular stimulating hormone (FSH). At the end of the experiment, body weight, testes weight and epididymal sperm parameters was studied. Furthermore, histo-morphometric and histopathological study changes were examined. The results revealed a significant decrease in testes weight to body weight ratio, serum testosterone, sperm concentration and motility, diameters of seminiferous tubules, height of seminiferous epithelia and number of Leydig cells, moreover the results showed a significant increase in serum FSH, dead sperm and abnormal sperm morphology in group T1 when compared with the other groups. Comparing to lead acetate treated rats, group T3 showed an improvement at the level of the studied parameters, accompanied with mild congestion in the interstitial tissue with a marked developing proliferation of spermatogenic cells, as well as presence of mature spermatozoa in the seminiferous tubules. In conclusion, subchronic exposure of rats to lead acetate showed an amelioration of all reproductive parameters near to normal values due to the antioxidant effects of ALA and the histological changes of the testes confirmed such change in serum parameters and the beneficial role of ALA.