Iraqi Journal of Veterinary Sciences

This study aimed to investigate the dose-dependent and time-dependent effect of magnetic iron oxide nanoparticles (MIONPs) on reproduction in male rats. Following their synthesis, the physicochemical properties of MIONPs were determined. Sixty-four adult male rats, aged 90 days were randomly assigned to control (C), orally administered with distilled water, and three treated groups, orally administered with 1, 5, 10 mg/kg/day of MIONPs solution (TL, TM, and TH groups, respectively), for 28 days. Each group was allocated to two subgroups, sacrificed after 14 and 28 days of treatment. After each period, the males were weighed and sacrificed. Decreased body weight and genital organ weights were shown in TM and TH groups, at both experimental periods, compared with control in a dose-dependent manner. The serum concentration of GnRH, FSH, LH, and testosterone increased in the TL group and decreased in TM and TH groups compared with control in a dose-dependent and time-dependent manner. At both periods, the lowest expression levels of pituitary FSHβ and LHβ genes and testicular inh-α and LHR genes were recorded in TM and TH groups, and the highest levels were expressed in the TL group. The testis sections of TL males, showed normal architecture, but those from TM and TH groups showed degenerative and necrotic changes, reduced germinal epithelium, vacuolation, and decreased number of spermatocytes apparent. It is concluded that a low dose of MIONPS has a beneficial effect, whereas moderate or high doses have pathological effects on male reproduction.

This study was carried out to investigate the effect of induced prepubertal hyperthyroidism on the reproductive functions of male rats at the pubertal stage. Hyperthyroidism was induced by supplementing thyroxin in drinking water (0.002% w/v) and drenching of 200 μg/kg body weight. Sixty immature males (aged 50 days) were allocated to control and hyperthyroid (PH) groups, administered with distilled water and thyroxin, respectively. Each group was subdivided into three subgroups, sacrificed after 15 days (C15 and PH15), after administration for 15 days and left without treatment for 15 days (C15+ and PH15+), or after 30 days (C30 and PH30). After each period, body weight and relative weight of genital organs were recorded. Serum concentrations of thyroid stimulating hormone (TSH), thyroxin (TT4), triiodothyronine (TT3), follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone was assessed. The expression levels of testicular inha and thyroid hormone receptor (THR) genes were analyzed. Histopathological examination of testis was studied. Compared with control, PH group male rats showed decreased body weight gain and genital organ weights at all experimental periods, increased levels of serum TT4, TT3, and LH, decreased levels of TSH, FSH, and testosterone, and lower expression levels of testicular inha and THR genes. Testicular sections of PH group male rats, showed reduced germinal epithelium, vacuolation, and decreased the number of spermatocytes and Sertoli cells compared with control. In conclusion, the disturbed fluctuations of sex steroid hormones due to prepubertal hyperthyroidism might cause retardation of the testes’ development. 

The goal of our research is to evaluate the histological impact of nano ZnO particles (NZnO) on the testis and epididymis of adult male rabbits treated with I.P of 600mg/kg body weight of nano ZnO particles twice a week for three weeks and to the protective effect of vitamin E versus the effect of nano zinc oxide particles. Twenty-four adult male rabbits were used in this research and divided into four groups. The first group was the control group injected with intraperitoneal distal water, and the second group was injected with 600mg/kg body weight of nano Zno particles I.P. twice a week for three weeks, the third group was injected with 600 mg/kg body weight of nano Zno particles I.P. and co-administrated with 100 mg/kg body weight of vitamin e orally, while the fourth group received 100mg/kg body weight of vitamin e orally. The histological results showed that the nano ZnO particles treatment causes noticeable changes in the testis and epididymis. These changes are characterized by thickening of tunica albuginea of testis, degenerative and necrotic changes of germ cells lining the seminiferous tubules, arrest of spermatogenesis, giant cell formation, degeneration, and necrosis of epithelial cells lining epididymis canals. The canals are free from sperms is observed. As for the group of animals treated with nano ZnO particles co-administrated with vitamin e showed improvement in the histological changes compared with the control and group treated with vitamin e only showed normal architecture of testis and epididymis. Moreover, there is a decrease in the level concentration of Testosterone of the animals treated with nano ZnO particles compared with other groups.

The current study aimed to determine the pathological effects of chronic poisoning with sodium fluoride on some reproductive organs like ovary, and testis of adult’s male and female albino rats. Thirty-six male and female adult’s albino rats were divided into six groups. The first and second group of male and female rats are control groups was given tap drinking water, the third and fourth groups of male and female rats was given 150 ppm of NaF, the fifth and sixth group of male and female rats was given 300 ppm of NaF respectively for 90 days. The weights of male and female genital were recorded. Histological exam of control groups of female rats showed the typical histological structure of the ovary, while the NaF treated groups showed a decrease in growing follicles, in addition to thickening in tunica albuginea and deposition of eosinophilic material. In male control groups, the sections showed the typical histological structures of the testis, while the treated groups showed multinucleated spermatids in addition to the deposition of amorphous eosinophilic material in the interstitial tissue, coagulative necrosis, in addition to apoptotic and sloughed spermatogonia in the lumen of seminiferous tubule. These results indicate that sodium fluoride with 300 ppm has toxic effects on organ body weights and on the histology of the gonads of adult’s male and female albino rats.

The present study aimed to investigate the histological changes of Ganoderma lucidum (Red Reishi mushroom) on female and male reproductive system by using light microscope. Fifteen white rat weighing 200-250g was used in the experiments. The animals was divided mainly into three groups and these groups were subdivided into 3 groups for female, and 3 groups for male, that’s group represented as control group without treatment (G1). The 2nd received only 0.03 gm and vitamin C diluted with D.W. and the 3rd group received 0.03 gm of Ganoderma lucidum . The results of the present study showed that there are no side efects of Ganoderma lucidum on female and male genital system of rats (positive effects).